Source:http://linkedlifedata.com/resource/pubmed/id/12632066
Switch to
| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
4
|
| pubmed:dateCreated |
2003-3-12
|
| pubmed:abstractText |
The purpose of the present study was to examine the expression of interleukin (IL)-8 and IL-8 receptors and to evaluate the effects of IL-8 on human pancreatic cancer. We examined the expression of IL-8 and its two receptors (CXCR1 and CXCR2) in 40 surgically resected human pancreatic cancer tissues and in three different human pancreatic cancer cell lines (PANC-1, MIAPaCa-2 and Capan-2). The immunohistochemical analysis using specific antibodies demonstrated that positive staining for IL-8, CXCR1 and CXCR2 in surgically resected human pancreatic cancer was 50, 55 and 65%, respectively. Moreover, 40% of these cases were positive for both IL-8 and IL-8 receptors. In contrast, immunoreactive signals for those proteins were extremely suppressed in normal pancreatic tissues. All of the pancreatic cancer cell lines expressed IL-8 and IL-8 receptors at the RNA and protein levels. Receptor binding experiments using 125I-labeled IL-8 showed that PANC-1 cells had specific binding sites for IL-8. The cell proliferation assay demonstrated that IL-8 did not affect the growth of the three cell lines. However, treatment with IL-8 enhanced the invasiveness into Matrigel and increased the activity of matrix metalloproteinase (MMP)-2 in supernatants of the PANC-1 cells. These results demonstrate that IL-8 and IL-8 receptors are over-expressed in pancreatic cancer, and suggest that IL-8 regulates MMP-2 activity and plays an important role in the invasiveness of human pancreatic cancer.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Gelatin,
http://linkedlifedata.com/resource/pubmed/chemical/Interleukin-8,
http://linkedlifedata.com/resource/pubmed/chemical/Matrix Metalloproteinase 2,
http://linkedlifedata.com/resource/pubmed/chemical/RNA,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Interleukin-8A,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Interleukin-8B,
http://linkedlifedata.com/resource/pubmed/chemical/Recombinant Proteins
|
| pubmed:status |
MEDLINE
|
| pubmed:month |
Apr
|
| pubmed:issn |
1019-6439
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
22
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
765-71
|
| pubmed:dateRevised |
2006-11-15
|
| pubmed:meshHeading |
pubmed-meshheading:12632066-Binding Sites,
pubmed-meshheading:12632066-Blotting, Western,
pubmed-meshheading:12632066-Cell Division,
pubmed-meshheading:12632066-Cell Line, Tumor,
pubmed-meshheading:12632066-Dose-Response Relationship, Drug,
pubmed-meshheading:12632066-Gelatin,
pubmed-meshheading:12632066-Humans,
pubmed-meshheading:12632066-Immunohistochemistry,
pubmed-meshheading:12632066-Interleukin-8,
pubmed-meshheading:12632066-Matrix Metalloproteinase 2,
pubmed-meshheading:12632066-Neoplasm Invasiveness,
pubmed-meshheading:12632066-Pancreatic Neoplasms,
pubmed-meshheading:12632066-Precipitin Tests,
pubmed-meshheading:12632066-Protein Binding,
pubmed-meshheading:12632066-RNA,
pubmed-meshheading:12632066-Receptors, Interleukin-8A,
pubmed-meshheading:12632066-Receptors, Interleukin-8B,
pubmed-meshheading:12632066-Recombinant Proteins,
pubmed-meshheading:12632066-Reverse Transcriptase Polymerase Chain Reaction
|
| pubmed:year |
2003
|
| pubmed:articleTitle |
Potential involvement of IL-8 and its receptors in the invasiveness of pancreatic cancer cells.
|
| pubmed:affiliation |
Department of Medicine and Molecular Science, Hiroshima University Graduate School of Biomedical Sciences, Hiroshima 734-8551, Japan. ykuwada@hiroshima-u.ac.jp
|
| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|