Linked Life Data

12542859

Source:http://linkedlifedata.com/resource/pubmed/id/12542859

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
12
pubmed:dateCreated
2003-2-7
pubmed:abstractText
Mitogen-activated protein kinases (MAPK) play a crucial role in signal transduction that regulates gene expression through transcriptional factor activity. The purpose of this study was to investigate the temporal expression and topographic distribution of the activated MAPK pathways including extracellular signal-regulated protein kinase (ERK), c-Jun NH(2)-terminal kinase (JNK), and p38 MAPK following traumatic brain injury (TBI) in the cortex of the rat brain. Adult male Sprague-Dawley rats (300-400 g) were subjected to lateral fluid percussion injury of moderate severity (3.5-4.0 atm) using the Dragonfly device model (no. HPD-1700). Phosphorylated-MAPK protein levels were quantified using Western blot analysis. Topographic distribution of immunoreactivity for phosphorylated-MAPK was examined using immunohistochemistry. Our findings showed that TBI significantly increased the phosphorylated-ERK (p-ERK) and -JNK (p-JNK) levels, but not the -p38 (p-p38) protein levels in the cortex surrounding the injury site. The immunoreactivity for p-ERK and p-JNK immediately after TBI were localized in neurons. The immunoreactivity for p-JNK was uniformly but only transiently induced and returned to control levels 1 h after TBI. The immunoreactivity for p-ERK was confirmed up until 30 min after TBI in the superficial neuronal layers. Double immunostaining using a glial-specific marker demonstrated that p-ERK was prominent in astrocytes 6 h after TBI. The current results suggest that the ERK and JNK pathways, but not the p38 MAPK pathways are involved in signal transduction in the cortex following TBI.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Dec
pubmed:issn
0897-7151
pubmed:author
pubmed:issnType
Print
pubmed:volume
19
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1587-96
pubmed:dateRevised
2009-11-19
pubmed:meshHeading
pubmed-meshheading:12542859-Animals, pubmed-meshheading:12542859-Blotting, Western, pubmed-meshheading:12542859-Brain Injuries, pubmed-meshheading:12542859-Cerebral Cortex, pubmed-meshheading:12542859-Glial Fibrillary Acidic Protein, pubmed-meshheading:12542859-Immunohistochemistry, pubmed-meshheading:12542859-JNK Mitogen-Activated Protein Kinases, pubmed-meshheading:12542859-MAP Kinase Kinase 4, pubmed-meshheading:12542859-MAP Kinase Signaling System, pubmed-meshheading:12542859-Male, pubmed-meshheading:12542859-Mitogen-Activated Protein Kinase Kinases, pubmed-meshheading:12542859-Mitogen-Activated Protein Kinases, pubmed-meshheading:12542859-Percussion, pubmed-meshheading:12542859-Rats, pubmed-meshheading:12542859-Rats, Sprague-Dawley, pubmed-meshheading:12542859-Time Factors, pubmed-meshheading:12542859-eIF-2 Kinase, pubmed-meshheading:12542859-p38 Mitogen-Activated Protein Kinases
pubmed:year
2002
pubmed:articleTitle
Temporal and spatial profile of phosphorylated mitogen-activated protein kinase pathways after lateral fluid percussion injury in the cortex of the rat brain.
pubmed:affiliation
Department of Neurosurgery, National Defense Medical College, Tokorozawa, Japan. grd1405@gr.ndmc.ac.jp
pubmed:publicationType
Journal Article, Comparative Study