12496105

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Predicate	Object
rdf:type	pubmed:Citation
lifeskim:mentions	umls-concept:C0012222, umls-concept:C0023768, umls-concept:C0205195, umls-concept:C0242849, umls-concept:C0449445, umls-concept:C1138405, umls-concept:C1521721
pubmed:issue	6
pubmed:dateCreated	2002-12-23
pubmed:abstractText	Proteins and other macromolecules are believed to hinder molecular lateral diffusion in cellular membranes. We have constructed a well-characterized model system to better understand how obstacles in lipid bilayers obstruct diffusion. Fluorescence recovery after photobleaching was used to measure the lateral diffusion coefficient in single supported bilayers composed of mixtures of 1,2-dilauroylphosphotidylcholine (DLPC) and 1,2-distearoylphosphotidylcholine (DSPC). Because these lipids are immiscible and phase separate at room temperature, a novel quenching technique allowed us to construct fluid DLPC bilayers containing small disk-shaped gel-phase DSPC domains that acted as obstacles to lateral diffusion. Our experimental setup enabled us to analyze the same samples with atomic force microscopy and exactly characterize the size, shape, and number of gel-phase domains before measuring the obstacle-dependent diffusion coefficient. Lateral obstructed diffusion was found to be dependent on obstacle area fraction, size, and geometry. Analysis of our results using a free area diffusion model shows the possibility of unexpected long-range ordering of fluid-phase lipids around the gel-phase obstacles. This lipid ordering has implications for lipid-mediated protein interactions in cellular membranes.
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pubmed:language	eng
pubmed:journal	http://linkedlifedata.com/resource/pubmed/journal/0370626
pubmed:citationSubset	IM
pubmed:chemical	http://linkedlifedata.com/resource/pubmed/chemical/1,2-distearoyllecithin, http://linkedlifedata.com/resource/pubmed/chemical/1,2-linoleoylphosphatidylcholine, http://linkedlifedata.com/resource/pubmed/chemical/Gels, http://linkedlifedata.com/resource/pubmed/chemical/Lipid Bilayers, http://linkedlifedata.com/resource/pubmed/chemical/Membranes, Artificial, http://linkedlifedata.com/resource/pubmed/chemical/Phosphatidylcholines
pubmed:status	MEDLINE
pubmed:month	Dec
pubmed:issn	0006-3495
pubmed:author	pubmed-author:LongoMarjorie LML, pubmed-author:RattoTimothy VTV
pubmed:issnType	Print
pubmed:volume	83
pubmed:owner	NLM
pubmed:authorsComplete	Y
pubmed:pagination	3380-92
pubmed:dateRevised	2010-9-14
pubmed:meshHeading	pubmed-meshheading:12496105-Cell Membrane, pubmed-meshheading:12496105-Diffusion, pubmed-meshheading:12496105-Fluorescence Recovery After Photobleaching, pubmed-meshheading:12496105-Gels, pubmed-meshheading:12496105-Lipid Bilayers, pubmed-meshheading:12496105-Membrane Fluidity, pubmed-meshheading:12496105-Membranes, Artificial, pubmed-meshheading:12496105-Microscopy, Atomic Force, pubmed-meshheading:12496105-Motion, pubmed-meshheading:12496105-Phosphatidylcholines
pubmed:year	2002
pubmed:articleTitle	Obstructed diffusion in phase-separated supported lipid bilayers: a combined atomic force microscopy and fluorescence recovery after photobleaching approach.
pubmed:affiliation	Biophysics Graduate Group, Division of Biological Sciences, University of California-Davis, One Shields Avenue, Davis, CA 95616, USA.
pubmed:publicationType	Journal Article, Research Support, U.S. Gov't, Non-P.H.S., Research Support, Non-U.S. Gov't, Evaluation Studies