Fluorescence Recovery After Photobleaching

Source:http://linkedlifedata.com/resource/umls/id/C1138405

MSH: A method used to study the lateral movement of MEMBRANE PROTEINS and LIPIDS. A small area of a cell membrane is bleached by laser light and the amount of time necessary for unbleached fluorescent marker-tagged proteins to diffuse back into the bleached site is a measurement of the cell membrane's fluidity. The diffusion coefficient of a protein or lipid in the membrane can be calculated from the data. (From Segen, Current Med Talk, 1995).,CSP: method used to study lateral movements of membrane proteins and lipids; a small area of a cell membrane is bleached by laser light and the amount of time necessary for unbleached fluorescent marker tagged proteins to diffuse back into the bleached site is a measurement of the cell membrane's fluidity.

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