Source:http://linkedlifedata.com/resource/pubmed/id/12456995
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:dateCreated |
2002-11-28
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| pubmed:abstractText |
RNA differential display (DD) RT-PCR is a useful method to identify and clone differentially expressed genes. However, the rate of false positives and redundancy associated with this PCR-based method as well as laborious downstream screening steps constitute major limitations. Here we present DD RT-PCR and reverse northern (RN) protocols allowing rapid and acurate identification of genes upregulated in porcine endothelial cells (EC) in response to TNFalpha.
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| pubmed:language |
eng
|
| pubmed:citationSubset |
IM
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| pubmed:chemical | |
| pubmed:status |
MEDLINE
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| pubmed:author | |
| pubmed:owner |
NLM
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| pubmed:authorsComplete |
Y
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| pubmed:pagination |
559-67
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| pubmed:dateRevised |
2009-11-3
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| pubmed:articleTitle |
Rapid selection of differentially expressed genes in TNF[alpha]-activated endothelial cells.
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| pubmed:affiliation |
Institut National de la Recherche Médicale and Institut de Transplantation et de Recherche en Transplantation, Nantes, FR.
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