Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
23
pubmed:dateCreated
2002-11-11
pubmed:abstractText
The C30 carotene synthase CrtM from Staphylococcus aureus and the C40 carotene synthase CrtB from Erwinia uredovora were swapped into their respective foreign C40 and C30 biosynthetic pathways (heterologously expressed in Escherichia coli) and evaluated for function. Each displayed negligible ability to synthesize the natural carotenoid product of the other. After one round of mutagenesis and screening, we isolated 116 variants of CrtM able to synthesize C40 carotenoids. In contrast, we failed to find a single variant of CrtB with detectable C30 activity. Subsequent analysis revealed that the best CrtM mutants performed comparably to CrtB in an in vivo C40 pathway. These mutants showed significant variation in performance in their original C30 pathway, indicating the emergence of enzymes with broadened substrate specificity as well as those with shifted specificity. We discovered that Phe 26 alone determines the specificity of CrtM. The plasticity of CrtM with respect to its substrate and product range highlights the potential for creating further new carotenoid backbone structures.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/12426357-10467173, http://linkedlifedata.com/resource/pubmed/commentcorrection/12426357-10880976, http://linkedlifedata.com/resource/pubmed/commentcorrection/12426357-10888843, http://linkedlifedata.com/resource/pubmed/commentcorrection/12426357-10896663, http://linkedlifedata.com/resource/pubmed/commentcorrection/12426357-10932152, http://linkedlifedata.com/resource/pubmed/commentcorrection/12426357-10972818, http://linkedlifedata.com/resource/pubmed/commentcorrection/12426357-11027152, http://linkedlifedata.com/resource/pubmed/commentcorrection/12426357-11048954, http://linkedlifedata.com/resource/pubmed/commentcorrection/12426357-11092891, http://linkedlifedata.com/resource/pubmed/commentcorrection/12426357-11098133, http://linkedlifedata.com/resource/pubmed/commentcorrection/12426357-11226339, http://linkedlifedata.com/resource/pubmed/commentcorrection/12426357-11437362, http://linkedlifedata.com/resource/pubmed/commentcorrection/12426357-11526111, http://linkedlifedata.com/resource/pubmed/commentcorrection/12426357-11566453, http://linkedlifedata.com/resource/pubmed/commentcorrection/12426357-11943208, http://linkedlifedata.com/resource/pubmed/commentcorrection/12426357-1400305, http://linkedlifedata.com/resource/pubmed/commentcorrection/12426357-1438319, http://linkedlifedata.com/resource/pubmed/commentcorrection/12426357-182217, http://linkedlifedata.com/resource/pubmed/commentcorrection/12426357-1995611, http://linkedlifedata.com/resource/pubmed/commentcorrection/12426357-2849754, http://linkedlifedata.com/resource/pubmed/commentcorrection/12426357-4212486, http://linkedlifedata.com/resource/pubmed/commentcorrection/12426357-6387426, http://linkedlifedata.com/resource/pubmed/commentcorrection/12426357-7873527, http://linkedlifedata.com/resource/pubmed/commentcorrection/12426357-8002598, http://linkedlifedata.com/resource/pubmed/commentcorrection/12426357-8086404, http://linkedlifedata.com/resource/pubmed/commentcorrection/12426357-8484753, http://linkedlifedata.com/resource/pubmed/commentcorrection/12426357-8626566, http://linkedlifedata.com/resource/pubmed/commentcorrection/12426357-8635686, http://linkedlifedata.com/resource/pubmed/commentcorrection/12426357-8641556, http://linkedlifedata.com/resource/pubmed/commentcorrection/12426357-8645170, http://linkedlifedata.com/resource/pubmed/commentcorrection/12426357-8692906, http://linkedlifedata.com/resource/pubmed/commentcorrection/12426357-8702542, http://linkedlifedata.com/resource/pubmed/commentcorrection/12426357-8823172, http://linkedlifedata.com/resource/pubmed/commentcorrection/12426357-8940054, http://linkedlifedata.com/resource/pubmed/commentcorrection/12426357-8986756, http://linkedlifedata.com/resource/pubmed/commentcorrection/12426357-9030588, http://linkedlifedata.com/resource/pubmed/commentcorrection/12426357-9204881, http://linkedlifedata.com/resource/pubmed/commentcorrection/12426357-9297464, http://linkedlifedata.com/resource/pubmed/commentcorrection/12426357-9298207, http://linkedlifedata.com/resource/pubmed/commentcorrection/12426357-9324768, http://linkedlifedata.com/resource/pubmed/commentcorrection/12426357-9371761, http://linkedlifedata.com/resource/pubmed/commentcorrection/12426357-9442047, http://linkedlifedata.com/resource/pubmed/commentcorrection/12426357-9470222, http://linkedlifedata.com/resource/pubmed/commentcorrection/12426357-9603990, http://linkedlifedata.com/resource/pubmed/commentcorrection/12426357-9636041, http://linkedlifedata.com/resource/pubmed/commentcorrection/12426357-9914250, http://linkedlifedata.com/resource/pubmed/commentcorrection/12426357-9927721
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Dec
pubmed:issn
0021-9193
pubmed:author
pubmed:issnType
Print
pubmed:volume
184
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
6690-9
pubmed:dateRevised
2010-9-14
pubmed:meshHeading
pubmed:year
2002
pubmed:articleTitle
Evolution of the C30 carotenoid synthase CrtM for function in a C40 pathway.
pubmed:affiliation
Department of Chemical Engineering, California Institute of Technology 210-41, Pasadena, California 91125, USA. umeno@cheme.caltech.edu
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, Non-P.H.S., Research Support, Non-U.S. Gov't