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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
1-3
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pubmed:dateCreated |
2002-10-31
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pubmed:abstractText |
The effects of pituitary adenylate cyclase activating polypeptide (PACAP) on human lung cancer cell line NCI-1299 mitogen activated protein kinase (MAPK) tyrosine phosphorylation and vascular endothelial cell growth factor (VEGF) expression were investigated. PACAP-27 (100 nM) increased MAPK tyrosine phosphorylation 3-fold, 5 min after addition to NCI-H1299 cells. PACAP caused tyrosine phosphorylation in a concentration-dependent manner being half-maximal at 10 nM PACAP-27. PACAP-27 or PACAP-38 (100 nM) but not PACAP28-38 or VIP caused increased MAPK tyrosine phosphorylation using NCI-H1299 cells. Also, the increase in MAPK tyrosine phosphorylation caused by PACAP-27 was totally inhibited by 10 microM PACAP(6-38), a PAC(1) receptor antagonist or 10 microM PD98059, a MAPKK inhibitor. These results suggest that PAC(1) receptors regulate tyrosine phosphorylation of MAPK in a MAPKK-dependent manner. PACAP-27 (100 nM) caused increased VEGF mRNA in NCI-H1299 cells after 8 h. The increase in VEGF mRNA caused by PACAP-27 was partially inhibited by PACAP(6-38), PD98059 and H-89. Addition of VIP to NCI-H1299 cells caused increased VEGF mRNA, which was totally inhibited by H89, a PKA inhibitor. These results suggest that PAC(1) and VPAC(1) receptors regulate VEGF expression in lung cancer cells.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/ADCYAP1 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Adcyap1 protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/Endothelial Growth Factors,
http://linkedlifedata.com/resource/pubmed/chemical/Intercellular Signaling Peptides...,
http://linkedlifedata.com/resource/pubmed/chemical/Lymphokines,
http://linkedlifedata.com/resource/pubmed/chemical/Mitogen-Activated Protein Kinases,
http://linkedlifedata.com/resource/pubmed/chemical/Neuropeptides,
http://linkedlifedata.com/resource/pubmed/chemical/Phosphotyrosine,
http://linkedlifedata.com/resource/pubmed/chemical/Pituitary Adenylate...,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger,
http://linkedlifedata.com/resource/pubmed/chemical/Vascular Endothelial Growth Factor A,
http://linkedlifedata.com/resource/pubmed/chemical/Vascular Endothelial Growth Factors
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pubmed:status |
MEDLINE
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pubmed:month |
Nov
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pubmed:issn |
0167-0115
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
15
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pubmed:volume |
109
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
135-40
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pubmed:dateRevised |
2009-11-19
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pubmed:meshHeading |
pubmed-meshheading:12409225-3T3 Cells,
pubmed-meshheading:12409225-Animals,
pubmed-meshheading:12409225-Endothelial Growth Factors,
pubmed-meshheading:12409225-Humans,
pubmed-meshheading:12409225-Intercellular Signaling Peptides and Proteins,
pubmed-meshheading:12409225-Lung Neoplasms,
pubmed-meshheading:12409225-Lymphokines,
pubmed-meshheading:12409225-Mice,
pubmed-meshheading:12409225-Mitogen-Activated Protein Kinases,
pubmed-meshheading:12409225-Neuropeptides,
pubmed-meshheading:12409225-Phosphorylation,
pubmed-meshheading:12409225-Phosphotyrosine,
pubmed-meshheading:12409225-Pituitary Adenylate Cyclase-Activating Polypeptide,
pubmed-meshheading:12409225-RNA, Messenger,
pubmed-meshheading:12409225-Transfection,
pubmed-meshheading:12409225-Tumor Cells, Cultured,
pubmed-meshheading:12409225-Vascular Endothelial Growth Factor A,
pubmed-meshheading:12409225-Vascular Endothelial Growth Factors
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pubmed:year |
2002
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pubmed:articleTitle |
PACAP-27 tyrosine phosphorylates mitogen activated protein kinase and increases VEGF mRNAs in human lung cancer cells.
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pubmed:affiliation |
Center for Cancer Research, National Cancer Institute, Bethesda, MD 20892, USA. moodyt@mail.nih.gov
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pubmed:publicationType |
Journal Article
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