Source:http://linkedlifedata.com/resource/pubmed/id/12054360
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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
4
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pubmed:dateCreated |
2002-6-10
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pubmed:abstractText |
An online automatic sample cleanup system was developed for use with electrospray ionization tandem mass spectrometry (ESI-MS-MS) for the quantitative detection of the benzene exposure biomarker S-phenylmercapturic acid (S-PMA) in human urine. The sample clean-up system was constructed with an autosampling device, a reversed-phase C18 trap cartridge, a two-position switching valve, and controlling computer software and hardware. The sample cleanup system was interfaced directly with the ESI source of a triple-stage-quadrupole MS using multiple reaction monitoring of negative product ions derived from S-PMA and the internal standard as the detection mode. The calibration curve was linear using human urine spiked at concentrations from 0.23 to 100 mg/L S-PMA (R2 = 0.997). The detection limit of the analytical system for neat S-PMA standard solution was 0.04 microg/L, whereas the detection limit was estimated to be lower than 0.35 microg/L for a urine matrix containing trace amounts of S-PMA. Without tedious manual sample cleanup procedures, the analytical system is fully automatic and therefore useful for high-throughput urinary S-PMA determination. With the selectivity and the sensitivity provided by MS-MS detection, the analytical system can be used for high-throughput and accurate determination of S-PMA levels in human urinary samples as a biomarker for benzene exposure.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Acetylcysteine,
http://linkedlifedata.com/resource/pubmed/chemical/Air Pollutants, Occupational,
http://linkedlifedata.com/resource/pubmed/chemical/Benzene,
http://linkedlifedata.com/resource/pubmed/chemical/Biological Markers,
http://linkedlifedata.com/resource/pubmed/chemical/S-phenyl-N-acetylcysteine
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pubmed:status |
MEDLINE
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pubmed:issn |
0146-4760
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
26
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
205-10
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pubmed:dateRevised |
2009-11-19
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pubmed:meshHeading |
pubmed-meshheading:12054360-Acetylcysteine,
pubmed-meshheading:12054360-Air Pollutants, Occupational,
pubmed-meshheading:12054360-Automation,
pubmed-meshheading:12054360-Benzene,
pubmed-meshheading:12054360-Biological Markers,
pubmed-meshheading:12054360-Chemistry Techniques, Analytical,
pubmed-meshheading:12054360-Humans,
pubmed-meshheading:12054360-Inhalation Exposure,
pubmed-meshheading:12054360-Occupational Exposure,
pubmed-meshheading:12054360-Sensitivity and Specificity,
pubmed-meshheading:12054360-Specimen Handling,
pubmed-meshheading:12054360-Spectrometry, Mass, Electrospray Ionization
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pubmed:articleTitle |
An online automatic sample cleanup system for the quantitative detection of the benzene exposure biomarker S-phenylmercapturic acid in human urine by electrospray ionization tandem mass spectrometry.
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pubmed:affiliation |
Department of Environmental and Occupational Health, National Cheng Kung University College of Medicine, Tainan, Taiwan. liaopc@mail.ncku.edu.tw
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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