Linked Life Data

11979338

Source:http://linkedlifedata.com/resource/pubmed/id/11979338

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
5
pubmed:dateCreated
2002-4-29
pubmed:abstractText
Osteopontin (OPN), a secreted phosphoprotein and chemotactic to monocytes/macrophages, is upregulated in renal cortical tubules in a variety of rodent models of renal injury and is believed to possibly have a role in tubulointerstitial injury. We previously reported the establishment of a system for the quantification of messenger RNA (mRNA) expression in isolated rat glomeruli using laser-manipulated microdissection and real-time polymerase chain reaction. This system was applied to human renal biopsy specimens. We quantified OPN mRNA expression in proximal tubules of 5 patients with minimal change nephrotic syndrome (MCNS) and 11 patients with mild immunoglobulin A (IgA) glomerulonephritis. We also examined the correlation between OPN mRNA expression in proximal tubules and clinical data and pathological findings in glomeruli and tubulointerstitial regions. Patients with MCNS showed a positive correlation between OPN mRNA expression in proximal tubules and urinary protein excretion (r = 0.93; P < 0.05), whereas for patients with IgA glomerulonephritis, logarithmic values of OPN mRNA expression in proximal tubules positively correlated with low urinary protein levels (r = 0.72; P < 0.05). Pathological changes, ranging from nonexistent to minor, in glomeruli and tubulointerstitium of these patients with mild IgA glomerulonephritis did not significantly correlate with OPN mRNA expression in proximal tubules. In patients with mild IgA glomerulonephritis, OPN mRNA expression in proximal tubules increased exponentially in response to a small amount of urinary protein (<1.2 g/d).
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
May
pubmed:issn
1523-6838
pubmed:author
pubmed:copyrightInfo
Copyright 2002 by the National Kidney Foundation, Inc.
pubmed:issnType
Electronic
pubmed:volume
39
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
948-57
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
2002
pubmed:articleTitle
Quantitative analyses of osteopontin mRNA expression in human proximal tubules isolated from renal biopsy tissue sections of minimal change nephrotic syndrome and IgA glomerulonephropathy patients.
pubmed:affiliation
Department of Internal Medicine and Therapeutics, Graduate School of Medicine, Osaka University, Osaka, Japan.
pubmed:publicationType
Journal Article, Comparative Study, Research Support, Non-U.S. Gov't