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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
3
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pubmed:dateCreated |
2002-5-23
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pubmed:abstractText |
Transforming growth factor-beta (TGF-beta) and interleukin-4 (IL-4) have fibrogenic properties and induce extracellular matrix production in a variety of lung diseases. Connective tissue growth factor (CTGF) is a matrix signaling molecule stimulated by TGF-beta that in part mediates alpha1(I) collagen mRNA expression. In these studies, the regulation of CTGF expression by IL-4 in human lung fibroblasts was examined. Following 6 h of stimulation with IL-4, basal CTGF mRNA levels were unchanged as assessed by Northern blot analysis. However, IL-4 attenuated the TGF-beta-stimulated induction of CTGF mRNA expression by 50%. This effect was selective because IL-4 did not affect fibronectin or alpha1(I) collagen mRNA expression induced by TGF-beta. Experiments employing the transcriptional inhibitor actinomycin D suggest that IL-4 did not affect the stability of the CTGF mRNA. Transient transfection assays with 3TP-Lux, a luciferase gene controlled by a TGF-beta inducible promoter, and with a CTGF promoter construct indicate that IL-4 interfered with the TGF-beta-induced transcriptional activation of the CTGF gene.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/CTGF protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Collagen Type I,
http://linkedlifedata.com/resource/pubmed/chemical/Connective Tissue Growth Factor,
http://linkedlifedata.com/resource/pubmed/chemical/Dactinomycin,
http://linkedlifedata.com/resource/pubmed/chemical/Enzyme Inhibitors,
http://linkedlifedata.com/resource/pubmed/chemical/Fibronectins,
http://linkedlifedata.com/resource/pubmed/chemical/Immediate-Early Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Insulin,
http://linkedlifedata.com/resource/pubmed/chemical/Intercellular Signaling Peptides...,
http://linkedlifedata.com/resource/pubmed/chemical/Interleukin-4,
http://linkedlifedata.com/resource/pubmed/chemical/MAP Kinase Kinase Kinase 1,
http://linkedlifedata.com/resource/pubmed/chemical/MAP3K1 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Phosphatidylinositol 3-Kinases,
http://linkedlifedata.com/resource/pubmed/chemical/Protein-Serine-Threonine Kinases,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger,
http://linkedlifedata.com/resource/pubmed/chemical/Transforming Growth Factor beta
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pubmed:status |
MEDLINE
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pubmed:issn |
0730-2312
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pubmed:author |
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pubmed:copyrightInfo |
Copyright 2002 Wiley-Liss, Inc.
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pubmed:issnType |
Print
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pubmed:volume |
85
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
496-504
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pubmed:dateRevised |
2011-11-17
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pubmed:meshHeading |
pubmed-meshheading:11967989-Collagen Type I,
pubmed-meshheading:11967989-Connective Tissue Growth Factor,
pubmed-meshheading:11967989-Dactinomycin,
pubmed-meshheading:11967989-Dose-Response Relationship, Drug,
pubmed-meshheading:11967989-Enzyme Inhibitors,
pubmed-meshheading:11967989-Fibroblasts,
pubmed-meshheading:11967989-Fibronectins,
pubmed-meshheading:11967989-Gene Expression Regulation,
pubmed-meshheading:11967989-Humans,
pubmed-meshheading:11967989-Immediate-Early Proteins,
pubmed-meshheading:11967989-Insulin,
pubmed-meshheading:11967989-Intercellular Signaling Peptides and Proteins,
pubmed-meshheading:11967989-Interleukin-4,
pubmed-meshheading:11967989-Lung,
pubmed-meshheading:11967989-MAP Kinase Kinase Kinase 1,
pubmed-meshheading:11967989-Phosphatidylinositol 3-Kinases,
pubmed-meshheading:11967989-Promoter Regions, Genetic,
pubmed-meshheading:11967989-Protein-Serine-Threonine Kinases,
pubmed-meshheading:11967989-RNA, Messenger,
pubmed-meshheading:11967989-Transforming Growth Factor beta
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pubmed:year |
2002
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pubmed:articleTitle |
Interleukin-4 regulates connective tissue growth factor expression in human lung fibroblasts.
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pubmed:affiliation |
The Pulmonary Center, Boston University School of Medicine, Boston, Massachusetts 02118, USA. drishikof@lung.bumc.bu.edu
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, U.S. Gov't, Non-P.H.S.
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