Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
5
pubmed:dateCreated
2002-4-22
pubmed:abstractText
Myosin VI, an actin-based motor protein, and Disabled 2 (Dab2), a molecule involved in endocytosis and cell signalling, have been found to bind together using yeast and mammalian two-hybrid screens. In polarised epithelial cells, myosin VI is known to be associated with apical clathrin-coated vesicles and is believed to move them towards the minus end of actin filaments, away from the plasma membrane and into the cell. Dab2 belongs to a group of signal transduction proteins that bind in vitro to the FXNPXY sequence found in the cytosolic tails of members of the low-density lipoprotein receptor family. The central region of Dab2, containing two DPF motifs, binds to the clathrin adaptor protein AP-2, whereas a C-terminal region contains the binding site for myosin VI. This site is conserved in Dab1, the neuronal counterpart of Dab2. The interaction between Dab2 and myosin VI was confirmed by in vitro binding assays and coimmunoprecipitation and by their colocalisation in clathrin-coated pits/vesicles concentrated at the apical domain of polarised cells. These results suggest that the myosin VI-Dab2 interaction may be one link between the actin cytoskeleton and receptors undergoing endocytosis.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
May
pubmed:issn
1398-9219
pubmed:author
pubmed:issnType
Print
pubmed:volume
3
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
331-41
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed-meshheading:11967127-Actins, pubmed-meshheading:11967127-Adaptor Proteins, Signal Transducing, pubmed-meshheading:11967127-Adaptor Proteins, Vesicular Transport, pubmed-meshheading:11967127-Amino Acid Sequence, pubmed-meshheading:11967127-Animals, pubmed-meshheading:11967127-CHO Cells, pubmed-meshheading:11967127-Cell Line, pubmed-meshheading:11967127-Cricetinae, pubmed-meshheading:11967127-Cytoskeleton, pubmed-meshheading:11967127-Endocytosis, pubmed-meshheading:11967127-Genes, Tumor Suppressor, pubmed-meshheading:11967127-Humans, pubmed-meshheading:11967127-Molecular Sequence Data, pubmed-meshheading:11967127-Myosin Heavy Chains, pubmed-meshheading:11967127-Precipitin Tests, pubmed-meshheading:11967127-Protein Binding, pubmed-meshheading:11967127-Proteins, pubmed-meshheading:11967127-Rats, pubmed-meshheading:11967127-Sequence Homology, Amino Acid
pubmed:year
2002
pubmed:articleTitle
Myosin VI binds to and localises with Dab2, potentially linking receptor-mediated endocytosis and the actin cytoskeleton.
pubmed:affiliation
Fred Hutchinson Cancer Research Center, Division of Basic Sciences, 1100 Fairview Avenue North, Seattle, WA 98109-1024, USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't