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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
1
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pubmed:dateCreated |
2002-2-26
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pubmed:abstractText |
Introduction of the E1A early region of the human adenovirus type 5 impairs the ability of mammalian cells to arrest the cell cycle at G1/S after damage. Two-parameter fluorescent-activated cell sorting (FACS) with iododeoxyuridine revealed the radiation-induced G1/S arrest in rat embryo fibroblasts transformed with the complementing E1A + E1B-19 kDa oncogenes. This was due to selective inhibition of CycIE/Cdk2-associated kinase activity, while activities of type 2 kinase and of CyclA/Cdk2 complexes remained unchanged. The inhibitor of G1-phase cyclin kinases, p21/Waf1, was accumulated and interacted with target kinases both in normal and in transformed cells after irradiation. As shown by immunoprecipitation, p21/Waf1 formed complexes with the E1A on coproducts in the transformants, which possibly accounted for its functional inactivation. Kinase modification in cyclin-kinase complexes was assumed to play a key role in regulation of cyclin-dependent kinases in the transformants with inactivated p21/Waf1.
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pubmed:language |
rus
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Adenovirus E1A Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Adenovirus E1B Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/CDC2-CDC28 Kinases,
http://linkedlifedata.com/resource/pubmed/chemical/Cdk2 protein, rat,
http://linkedlifedata.com/resource/pubmed/chemical/Cdk4 protein, rat,
http://linkedlifedata.com/resource/pubmed/chemical/Cdkn1a protein, rat,
http://linkedlifedata.com/resource/pubmed/chemical/Cyclin A,
http://linkedlifedata.com/resource/pubmed/chemical/Cyclin E,
http://linkedlifedata.com/resource/pubmed/chemical/Cyclin-Dependent Kinase 2,
http://linkedlifedata.com/resource/pubmed/chemical/Cyclin-Dependent Kinase 4,
http://linkedlifedata.com/resource/pubmed/chemical/Cyclin-Dependent Kinase Inhibitor...,
http://linkedlifedata.com/resource/pubmed/chemical/Cyclin-Dependent Kinases,
http://linkedlifedata.com/resource/pubmed/chemical/Cyclins,
http://linkedlifedata.com/resource/pubmed/chemical/Protein-Serine-Threonine Kinases,
http://linkedlifedata.com/resource/pubmed/chemical/Proto-Oncogene Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Tumor Suppressor Protein p53
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pubmed:status |
MEDLINE
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pubmed:issn |
0026-8984
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
36
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
58-65
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pubmed:dateRevised |
2009-11-19
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pubmed:meshHeading |
pubmed-meshheading:11862714-Adenovirus E1A Proteins,
pubmed-meshheading:11862714-Adenovirus E1B Proteins,
pubmed-meshheading:11862714-Animals,
pubmed-meshheading:11862714-CDC2-CDC28 Kinases,
pubmed-meshheading:11862714-Cell Cycle,
pubmed-meshheading:11862714-Cells, Cultured,
pubmed-meshheading:11862714-Cyclin A,
pubmed-meshheading:11862714-Cyclin E,
pubmed-meshheading:11862714-Cyclin-Dependent Kinase 2,
pubmed-meshheading:11862714-Cyclin-Dependent Kinase 4,
pubmed-meshheading:11862714-Cyclin-Dependent Kinase Inhibitor p21,
pubmed-meshheading:11862714-Cyclin-Dependent Kinases,
pubmed-meshheading:11862714-Cyclins,
pubmed-meshheading:11862714-Embryo, Mammalian,
pubmed-meshheading:11862714-Fibroblasts,
pubmed-meshheading:11862714-Gamma Rays,
pubmed-meshheading:11862714-Precipitin Tests,
pubmed-meshheading:11862714-Protein-Serine-Threonine Kinases,
pubmed-meshheading:11862714-Proto-Oncogene Proteins,
pubmed-meshheading:11862714-Rats,
pubmed-meshheading:11862714-Transfection,
pubmed-meshheading:11862714-Tumor Suppressor Protein p53
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pubmed:articleTitle |
[Transfection with the E1A and E1B-19kDa oncogenes does not prevent rat embryo fibroblasts from cell cycle arrest after gamma-radiation].
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pubmed:affiliation |
Institute of Cytology, Russian Academy of Sciences, St. Petersburg, 194064 Russia.
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pubmed:publicationType |
Journal Article,
English Abstract
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