Linked Life Data

11710397

Source:http://linkedlifedata.com/resource/pubmed/id/11710397

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
2001-11-15
pubmed:abstractText
In young cucumber seedlings, the peg is a polar outgrowth of tissue that functions by snagging the seed coat, thereby freeing the cotyledons. The development of the peg is thought to be gravity-dependent and has become a model system for plant-gravity response. Peg development requires rapid cell expansion, a process thought to be catalyzed by alpha-expansins, and thus was a good system to identify expansins that were regulated by gravity. This study identified 7 new alpha-expansin cDNAs from cucumber seedlings (Cucumis sativus L. cv Burpee Hybrid II) and examined their expression patterns. Two alpha-expansins (CsExp3 and CsExp4) were more highly expressed in the peg and the root. Earlier reports stated that pegs tend not to form in the absence of gravity, so the expression levels were compared in the pegs of seedlings grown in space (STS-95), on a clinostat, and on earth (1 g). Pegs were observed to form at high frequency on clinostat and space-grown seedlings, yet on clinostats there was more than a 4-fold reduction in the expression of CsExp3 in the pegs of seedlings grown on clinostats vs. those grown at 1 g, while the CsExp4 gene appeared to be turned off (below detection limits). There were no detectable differences in expansin gene expression levels for the pegs of seedlings grown in space or in the orbiter environmental simulator (OES) (1 g) at NASA. The microgravity environment did not affect the expression of CsExp3 or CsExp4, and the clinostat did not simulate the microgravity environment well.
pubmed:keyword
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
S
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Oct
pubmed:issn
0031-9317
pubmed:author
pubmed:issnType
Print
pubmed:volume
113
pubmed:owner
NASA
pubmed:authorsComplete
Y
pubmed:pagination
292-300
pubmed:dateRevised
2008-3-10
pubmed:meshHeading
pubmed:year
2001
pubmed:articleTitle
The effect of a microgravity (space) environment on the expression of expansins from the peg and root tissues of Cucumis sativus.
pubmed:affiliation
Wisconsin Center for Space Automation and Robotics, The University of Wisconsin, Madison, WI 53706, USA. BMLink@facstaff.wisc.edu
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, Non-P.H.S.