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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
3
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pubmed:dateCreated |
2001-11-12
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pubmed:abstractText |
Segregation Distorter (SD) is a meiotic drive system in Drosophila that causes preferential transmission of the SD chromosome from SD/SD+ males owing to dysfunction of SD+ spermatids. The Sd locus, which is essential for distortion, encodes a truncated RanGAP (Ran GTPase activating protein), a key nuclear transport factor. Here, we show that Sd-RanGAP retains normal enzyme activity but is mislocalized to nuclei. Distortion is abolished when enzymatic activity or nuclear localization of Sd-RanGAP is perturbed. Overexpression of Ran or RanGEF (Ran GTPase exchange factor) in the male germline fully suppresses distortion. We conclude that mislocalization of Sd-RanGAP causes distortion by reducing nuclear RanGTP, thereby disrupting the Ran signaling pathway. Nuclear transport of a GFP reporter in salivary glands is impaired by SD, suggesting that a defect in nuclear transport may underlie sperm dysfunction.
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pubmed:commentsCorrections |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Sep
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pubmed:issn |
1534-5807
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
1
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
351-61
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:11702947-Active Transport, Cell Nucleus,
pubmed-meshheading:11702947-Animals,
pubmed-meshheading:11702947-Cell Nucleus,
pubmed-meshheading:11702947-Chromosome Segregation,
pubmed-meshheading:11702947-Drosophila Proteins,
pubmed-meshheading:11702947-Drosophila melanogaster,
pubmed-meshheading:11702947-Female,
pubmed-meshheading:11702947-GTPase-Activating Proteins,
pubmed-meshheading:11702947-Genes, Reporter,
pubmed-meshheading:11702947-Immunohistochemistry,
pubmed-meshheading:11702947-Insect Proteins,
pubmed-meshheading:11702947-Male,
pubmed-meshheading:11702947-Meiosis,
pubmed-meshheading:11702947-Recombinant Fusion Proteins,
pubmed-meshheading:11702947-Salivary Glands,
pubmed-meshheading:11702947-Spermatocytes,
pubmed-meshheading:11702947-Testis,
pubmed-meshheading:11702947-Transgenes
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pubmed:year |
2001
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pubmed:articleTitle |
Nuclear mislocalization of enzymatically active RanGAP causes segregation distortion in Drosophila.
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pubmed:affiliation |
Laboratory of Genetics, University of Wisconsin-Madison, 53706, USA.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, Non-P.H.S.
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