Linked Life Data

11540088

Source:http://linkedlifedata.com/resource/pubmed/id/11540088

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:dateCreated
1997-2-19
pubmed:abstractText
Mobile-phase additives, commonly used to modulate absorbate retention in gradient elution chromatography, are usually assumed to be either linearly retained or unretained. Previous theoretical work from our laboratory has shown that these modulators, such as salts in ion-exchange and hydrophobic interaction chromatography and organic modifiers in reversed-phase chromatography, can absorb nonlinearly, giving rise to gradient deformation. Consequently, adsorbate peaks that elute in the vicinity of the head of the deformed gradient may exhibit unusual shapes, form shoulders, and/or be concentrated. These effects for a reversed-phase sorbent with aqueous acetonitrile (ACN) as the modulator are verified experimentally. Gradient deformation is demonstrated experimentally and agrees with simulations based on ACN isotherm parameters that are independently determined from batch equilibrium studies using the layer model. Unusual absorbate peak shapes were found experimentally for single-component injections of phenylalanine, similar to those calculated by the simulations. A binary mixture of tryptophan and phenylalanine is used to demonstrate simultaneous concentration and separation, again in agreement with simulations. The possibility of gradient deformation in ion-exchange and hydrophobic interaction chromatography is discussed.
pubmed:grant
pubmed:keyword
pubmed:language
eng
pubmed:citationSubset
S
pubmed:chemical
pubmed:status
MEDLINE
pubmed:author
pubmed:owner
NASA
pubmed:authorsComplete
Y
pubmed:pagination
1184-93
pubmed:dateRevised
2008-3-14
pubmed:meshHeading
pubmed:articleTitle
Simultaneous concentration and purification through gradient deformation chromatography.
pubmed:affiliation
Laboratory of Renewable Resources Engineering, Purdue University, West Lafayette, IN 47907, USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, Non-P.H.S.