Linked Life Data

11536319

Source:http://linkedlifedata.com/resource/pubmed/id/11536319

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
5
pubmed:dateCreated
2001-9-5
pubmed:abstractText
Although excitotoxic and oxidative stress play important roles in spinal neuron death, the exact mechanism is not fully understood. We examined cell damage of primary culture of 11-day-old rat spinal cord by addition of glutamate, nitric oxide (NO) or peroxynitrite (PN) with detection of nitrotyrosine (NT) or terminal deoxynucleotidyl transferase-mediated dUTP-biotin in situ nick end labeling (TUNEL). With addition of glutamate, NOC18 (a slow NO releaser) or PN, immunoreactivity for NT became stronger in the cytoplasm of large motor neurons in the ventral horn at 6 to 48 hr and positive in the axons of the ventral horn at 24 to 48 hr. TUNEL positive nuclei were found in spinal large motor neurons from 24 hr, and the positive cell number greatly increased at 48 hr in contrast to the vehicle. Pretreatment of cultures with alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionic acid (AMPA)/kainate receptor antagonist, NO-suppressing agent, and antioxidant protected the immunoreactivity for NT or TUNEL. The present results suggest that both excitotoxic and oxidative stress play an important role in the upregulation of NT nitration and the apoptotic pathway in cultured rat spinal neurons.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Sep
pubmed:issn
0360-4012
pubmed:author
pubmed:copyrightInfo
Copyright 2001 Wiley-Liss, Inc.
pubmed:issnType
Print
pubmed:day
1
pubmed:volume
65
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
371-7
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed-meshheading:11536319-Animals, pubmed-meshheading:11536319-Antioxidants, pubmed-meshheading:11536319-Apoptosis, pubmed-meshheading:11536319-Cells, Cultured, pubmed-meshheading:11536319-Central Nervous System Diseases, pubmed-meshheading:11536319-Enzyme Inhibitors, pubmed-meshheading:11536319-Excitatory Amino Acid Antagonists, pubmed-meshheading:11536319-Free Radicals, pubmed-meshheading:11536319-Glutamic Acid, pubmed-meshheading:11536319-Immunohistochemistry, pubmed-meshheading:11536319-In Situ Nick-End Labeling, pubmed-meshheading:11536319-Interneurons, pubmed-meshheading:11536319-Motor Neurons, pubmed-meshheading:11536319-Nerve Degeneration, pubmed-meshheading:11536319-Neurons, pubmed-meshheading:11536319-Neurotoxins, pubmed-meshheading:11536319-Nitrates, pubmed-meshheading:11536319-Nitric Oxide Donors, pubmed-meshheading:11536319-Organ Culture Techniques, pubmed-meshheading:11536319-Oxidative Stress, pubmed-meshheading:11536319-Rats, pubmed-meshheading:11536319-Rats, Sprague-Dawley, pubmed-meshheading:11536319-Spinal Cord, pubmed-meshheading:11536319-Tyrosine
pubmed:year
2001
pubmed:articleTitle
Expressions of nitrotyrosine and TUNEL immunoreactivities in cultured rat spinal cord neurons after exposure to glutamate, nitric oxide, or peroxynitrite.
pubmed:affiliation
Department of Neurology, Graduate School of Medicine and Dentistry, Okayama University Medical School, Okayama, Japan. mambe@cc.okayama-u.ac.jp
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't