In Situ Nick-End Labeling

Source:http://linkedlifedata.com/resource/umls/id/C0600528

MSH: An in situ method for detecting areas of DNA which are nicked during APOPTOSIS. Terminal deoxynucleotidyl transferase is used to add labeled dUTP, in a template-independent manner, to the 3 prime OH ends of either single- or double-stranded DNA. The terminal deoxynucleotidyl transferase nick end labeling, or TUNEL, assay labels apoptosis on a single-cell level, making it more sensitive than agarose gel electrophoresis for analysis of DNA FRAGMENTATION.,CSP: an in situ method for detecting areas of DNA which are nicked during apoptosis; terminal deoxynucleotidyl transferase is used to add labeled dUTP, in a template-independent manner, to the 3 primer OH ends of either single- or double-stranded DNA; assay labels apoptosis on a single-cell level, making it more sensitive than agarose gel electrophoresis for analysis of DNA fragmentation.

Download in:

View as