Source:http://linkedlifedata.com/resource/pubmed/id/11319144
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
5
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| pubmed:dateCreated |
2001-4-25
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| pubmed:abstractText |
Cell division requires that cyclin-dependent kinases (Cdks) be activated by phosphorylation. In mitotic cells, this is accomplished by the Cdk-activating-kinase (CAK), which is a complex of cyclin H and Cdk7. There are currently no data on the role of CAK in meiotic cells. Previously, we have shown that cyclin A1 is meiosis-specific and forms an active kinase with Cdk2. Because cyclin A1 is required for meiosis, and its associated kinase must be phosphorylated (activated), we propose that cyclin H/Cdk7 function to activate cyclin A1/Cdk2 in meiotic cells. Here, we show that cyclin H and Cdk7 are present during meiosis. Using reverse transcription-polymerase chain reaction and in situ hybridization, we show that the mRNAs encoding cyclin H and Cdk7 are abundant in spermatocytes. Immunohistochemistry localized cyclin H and Cdk7 to the nucleus of spermatocytes in stages IV to XII of the spermatogenic cycle, overlapping the same stages that express cyclin A1-associated kinases. Finally, immunoprecipitation and histone H1-kinase assays of cyclin H and Cdk7 from testicular extracts show that these proteins interact to form an active kinase. We conclude that cyclin H/Cdk7 complexes are present and during meiosis, form active complexes in testicular cells and are strong candidates for the activating kinase for cyclin A1-associated kinase.
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| pubmed:grant | |
| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Ccnh protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/Cyclin H,
http://linkedlifedata.com/resource/pubmed/chemical/Cyclin-Dependent Kinases,
http://linkedlifedata.com/resource/pubmed/chemical/Cyclins,
http://linkedlifedata.com/resource/pubmed/chemical/Protein-Serine-Threonine Kinases,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger,
http://linkedlifedata.com/resource/pubmed/chemical/cyclin-dependent kinase-activating...
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| pubmed:status |
MEDLINE
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| pubmed:month |
May
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| pubmed:issn |
0006-3363
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| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
64
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| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
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| pubmed:pagination |
1400-8
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| pubmed:dateRevised |
2009-11-19
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| pubmed:meshHeading |
pubmed-meshheading:11319144-Animals,
pubmed-meshheading:11319144-Cell Nucleus,
pubmed-meshheading:11319144-Cloning, Molecular,
pubmed-meshheading:11319144-Cyclin H,
pubmed-meshheading:11319144-Cyclin-Dependent Kinases,
pubmed-meshheading:11319144-Cyclins,
pubmed-meshheading:11319144-Gene Expression,
pubmed-meshheading:11319144-Immunohistochemistry,
pubmed-meshheading:11319144-Immunosorbent Techniques,
pubmed-meshheading:11319144-In Situ Hybridization,
pubmed-meshheading:11319144-Male,
pubmed-meshheading:11319144-Meiosis,
pubmed-meshheading:11319144-Mice,
pubmed-meshheading:11319144-Mice, Mutant Strains,
pubmed-meshheading:11319144-Protein-Serine-Threonine Kinases,
pubmed-meshheading:11319144-RNA, Messenger,
pubmed-meshheading:11319144-Reverse Transcriptase Polymerase Chain Reaction,
pubmed-meshheading:11319144-Sertoli Cells,
pubmed-meshheading:11319144-Spermatogenesis,
pubmed-meshheading:11319144-Spermatozoa,
pubmed-meshheading:11319144-Testis
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| pubmed:year |
2001
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| pubmed:articleTitle |
Meiotic expression of the cyclin H/Cdk7 complex in male germ cells of the mouse.
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| pubmed:affiliation |
Department of Cell Biology & Biochemistry, Texas Tech University Health Sciences Center, Lubbock, Texas 79430, USA.
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| pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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