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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
9
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pubmed:dateCreated |
2001-4-11
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pubmed:abstractText |
In genetic vaccination, recipients are immunized with antigen-encoding nucleic acid, usually DNA. This study addressed the possibility of using the recombinant alpha virus RNA molecule, which replicates in the cytoplasm of transfected cells, as a novel approach for genetic vaccination. Mice were immunized with recombinant Semliki Forest virus RNA-encoding envelope proteins from one of 3 viruses: influenza A virus, a tickborne flavivirus (louping ill virus), or respiratory syncytial virus (RSV). Serologic analyses showed that antigen-specific antibody responses were elicited. IgG isotyping indicated that predominantly Th1 type immune responses were induced after immunization with RSV F protein-encoding RNA, which is relevant for protection against RSV infection. Challenge infection showed that RNA immunization had elicited significant levels of protection against the 3 model virus diseases.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
AIM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
May
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pubmed:issn |
0022-1899
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
1
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pubmed:volume |
183
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1395-8
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:11294672-Animals,
pubmed-meshheading:11294672-Encephalitis, Tick-Borne,
pubmed-meshheading:11294672-Encephalitis Viruses, Tick-Borne,
pubmed-meshheading:11294672-Female,
pubmed-meshheading:11294672-Immunity, Cellular,
pubmed-meshheading:11294672-Immunoglobulin G,
pubmed-meshheading:11294672-Influenza A virus,
pubmed-meshheading:11294672-Influenza Vaccines,
pubmed-meshheading:11294672-Mice,
pubmed-meshheading:11294672-Mice, Inbred BALB C,
pubmed-meshheading:11294672-Orthomyxoviridae Infections,
pubmed-meshheading:11294672-RNA, Viral,
pubmed-meshheading:11294672-Respiratory Syncytial Virus Infections,
pubmed-meshheading:11294672-Respiratory Syncytial Virus Vaccines,
pubmed-meshheading:11294672-Respiratory Syncytial Viruses,
pubmed-meshheading:11294672-Semliki forest virus,
pubmed-meshheading:11294672-Th1 Cells,
pubmed-meshheading:11294672-Transfection,
pubmed-meshheading:11294672-Vaccines, Synthetic,
pubmed-meshheading:11294672-Viral Vaccines
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pubmed:year |
2001
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pubmed:articleTitle |
Self-replicative RNA vaccines elicit protection against influenza A virus, respiratory syncytial virus, and a tickborne encephalitis virus.
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pubmed:affiliation |
Microbiology and Tumorbiology Center, Karolinska Institutet, S-171 77 Stockholm, Sweden.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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