Source:http://linkedlifedata.com/resource/pubmed/id/11254221
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
1
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| pubmed:dateCreated |
2001-3-19
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| pubmed:abstractText |
Peripheral blood mononuclear cells (PBMC) from 251 HIV-positive drug abusers of known clinical stage and from 40 healthy donors were tested for conventional immunologic markers (CD3, CD4, CD8, CD19, CD14, CD16/CD56, CD45 and HLA-DR). Additional cell parameters and the occurrence of spontaneous apoptosis (programmed cell death) were investigated on freshly isolated PBMC by flow cytometric measurement of either annexin-V bound to plasma membrane phosphatidylserine or propidium iodide uptake. The activity of gamma-glutamyltransferase (gamma-GT), an ectoenzyme contributing to the synthesis of the intracellular antioxidant glutathione (GSH) and involved in early apoptosis, was also determined in these cells. Immunocompetent T-cell counts were lower in HIV+ patients, with the exception of CD8+ and HLA-DR+ lymphocytes. The external binding of annexin-V was significantly higher in HIV+ PBMC and occurred in both CD8+ and CD4+ T-lymphocyte subsets. The activity of gamma-GT, was significantly lower in the PBMC from HIV+ patients, indicating that the redox status of PBMC may be affected in HIV+ individuals. Finally, the most dominant features characterising patients receiving antiretroviral therapy were greater long-term stability in the distribution of various cell parameters excepted the level of apoptosis.
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| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Annexin A5,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD,
http://linkedlifedata.com/resource/pubmed/chemical/Glutathione,
http://linkedlifedata.com/resource/pubmed/chemical/HLA-DR Antigens,
http://linkedlifedata.com/resource/pubmed/chemical/gamma-Glutamyltransferase
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| pubmed:status |
MEDLINE
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| pubmed:issn |
0921-8912
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| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
21
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| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
11-20
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| pubmed:dateRevised |
2006-11-15
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| pubmed:meshHeading |
pubmed-meshheading:11254221-Acquired Immunodeficiency Syndrome,
pubmed-meshheading:11254221-Adult,
pubmed-meshheading:11254221-Annexin A5,
pubmed-meshheading:11254221-Antigens, CD,
pubmed-meshheading:11254221-Apoptosis,
pubmed-meshheading:11254221-Cell Membrane Permeability,
pubmed-meshheading:11254221-Female,
pubmed-meshheading:11254221-Glutathione,
pubmed-meshheading:11254221-HIV Infections,
pubmed-meshheading:11254221-HLA-DR Antigens,
pubmed-meshheading:11254221-Humans,
pubmed-meshheading:11254221-Immunophenotyping,
pubmed-meshheading:11254221-Leukocytes, Mononuclear,
pubmed-meshheading:11254221-Male,
pubmed-meshheading:11254221-Middle Aged,
pubmed-meshheading:11254221-Oxidation-Reduction,
pubmed-meshheading:11254221-Oxidative Stress,
pubmed-meshheading:11254221-Severity of Illness Index,
pubmed-meshheading:11254221-Substance Abuse, Intravenous,
pubmed-meshheading:11254221-gamma-Glutamyltransferase
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| pubmed:year |
2000
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| pubmed:articleTitle |
Spontaneous apoptosis, oxidative status and immunophenotype markers in blood lymphocytes of AIDS patients.
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| pubmed:affiliation |
Laboratorio di Patologia Cellulare, Istituto Cantonale di Patologia, Locarno, Switzerland. glosa@guest.cscs.ch
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| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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