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rdf:type |
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lifeskim:mentions |
umls-concept:C0007634,
umls-concept:C0007745,
umls-concept:C0021467,
umls-concept:C0021469,
umls-concept:C0030685,
umls-concept:C0041536,
umls-concept:C0162638,
umls-concept:C0229671,
umls-concept:C0291573,
umls-concept:C0391871,
umls-concept:C0680255,
umls-concept:C1283071,
umls-concept:C1879547,
umls-concept:C1963578
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pubmed:issue |
2
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pubmed:dateCreated |
2001-3-2
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pubmed:abstractText |
Coenzyme Q10 (CoQ10) is a component of the antioxidant machinery that protects cell membranes from oxidative damage and decreases apoptosis in leukemic cells cultured in serum-depleted media. Serum deprivation induced apoptosis in CEM-C7H2 (CEM) and to a lesser extent in CEM-9F3, a subline overexpressing Bcl-2. Addition of CoQ10 to serum-free media decreased apoptosis in both cell lines. Serum withdrawal induced an early increase of neutral-sphingomyelinase activity, release of ceramide, and activation of caspase-3 in both cell lines, but this effect was more pronounced in CEM cells. CoQ10 prevented activation of this cascade of events. Lipids extracted from serum-depleted cultures activated caspase-3 independently of the presence of mitochondria in cell-free in vitro assays. Activation of caspase-3 by lipid extracts or ceramide was prevented by okadaic acid, indicating the implication of a phosphatase in this process. Our results support the hypothesis that plasma membrane CoQ10 regulate the initiation phase of serum withdrawal-induced apoptosis by preventing oxidative damage and thus avoiding activation of downstream effectors as neutral-sphingomyelinase and subsequent ceramide release and caspase activation pathways.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/CASP3 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Caspase 3,
http://linkedlifedata.com/resource/pubmed/chemical/Caspases,
http://linkedlifedata.com/resource/pubmed/chemical/Ceramides,
http://linkedlifedata.com/resource/pubmed/chemical/Coenzymes,
http://linkedlifedata.com/resource/pubmed/chemical/Culture Media, Serum-Free,
http://linkedlifedata.com/resource/pubmed/chemical/Electron Transport Complex IV,
http://linkedlifedata.com/resource/pubmed/chemical/Okadaic Acid,
http://linkedlifedata.com/resource/pubmed/chemical/Sphingomyelin Phosphodiesterase,
http://linkedlifedata.com/resource/pubmed/chemical/Ubiquinone,
http://linkedlifedata.com/resource/pubmed/chemical/coenzyme Q10
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pubmed:status |
MEDLINE
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pubmed:issn |
1523-0864
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
2
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
263-75
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pubmed:dateRevised |
2007-11-15
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pubmed:meshHeading |
pubmed-meshheading:11229531-Apoptosis,
pubmed-meshheading:11229531-Caspase 3,
pubmed-meshheading:11229531-Caspases,
pubmed-meshheading:11229531-Cell Membrane,
pubmed-meshheading:11229531-Cell-Free System,
pubmed-meshheading:11229531-Ceramides,
pubmed-meshheading:11229531-Coenzymes,
pubmed-meshheading:11229531-Culture Media, Serum-Free,
pubmed-meshheading:11229531-Electron Transport Complex IV,
pubmed-meshheading:11229531-Enzyme Activation,
pubmed-meshheading:11229531-Humans,
pubmed-meshheading:11229531-Leukemia,
pubmed-meshheading:11229531-Lipid Metabolism,
pubmed-meshheading:11229531-Mitochondria,
pubmed-meshheading:11229531-Okadaic Acid,
pubmed-meshheading:11229531-Oxidative Stress,
pubmed-meshheading:11229531-Sphingomyelin Phosphodiesterase,
pubmed-meshheading:11229531-Time Factors,
pubmed-meshheading:11229531-Tumor Cells, Cultured,
pubmed-meshheading:11229531-Ubiquinone
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pubmed:year |
2000
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pubmed:articleTitle |
Coenzyme Q protects cells against serum withdrawal-induced apoptosis by inhibition of ceramide release and caspase-3 activation.
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pubmed:affiliation |
Laboratorio Andaluz de Biología, Universidad Pablo de Olavide, Sevilla, Spain.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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