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rdf:type |
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lifeskim:mentions |
umls-concept:C0007586,
umls-concept:C0007634,
umls-concept:C0074993,
umls-concept:C0080129,
umls-concept:C0087111,
umls-concept:C0109317,
umls-concept:C0162638,
umls-concept:C0205263,
umls-concept:C0752312,
umls-concept:C1150579,
umls-concept:C1292733,
umls-concept:C1333340,
umls-concept:C1366882,
umls-concept:C1370600,
umls-concept:C1418975,
umls-concept:C1550548,
umls-concept:C1555714,
umls-concept:C1705654,
umls-concept:C1705767,
umls-concept:C1705791,
umls-concept:C1879547
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pubmed:issue |
2
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pubmed:dateCreated |
2001-2-22
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pubmed:abstractText |
We report that prosaposin treatment induced extracellular signal-regulated kinases (ERKs) and sphingosine kinase activity, increased DNA synthesis, and prevented cell apoptosis. Prosaposin treatment induced pheochromocytoma cells (PC12) to enter the S phase of the cell cycle; this effect was inhibited by the MEK inhibitor PD98059, indicating that prosaposin-induced ERK phosphorylation is required for stimulation of DNA synthesis. The prosaposin effect was also inhibited by pertussis toxin, indicating that the prosaposin receptor is a G-protein-coupled receptor. Prosaposin rescued PC12 cells from apoptosis induced by staurosporine or ceramide. Sphingosine kinase activity was increased by prosaposin treatment. We propose that this effect is a mechanism underlying the proliferative and anti-apoptotic functions of prosaposin. Prosaposin appears to be a key regulatory factor in the ceramide-S-1-P rheostat, which regulates cell fate.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/DNA, Neoplasm,
http://linkedlifedata.com/resource/pubmed/chemical/Enzyme Inhibitors,
http://linkedlifedata.com/resource/pubmed/chemical/Flavonoids,
http://linkedlifedata.com/resource/pubmed/chemical/Glycoproteins,
http://linkedlifedata.com/resource/pubmed/chemical/Mitogen-Activated Protein Kinases,
http://linkedlifedata.com/resource/pubmed/chemical/PD 98059,
http://linkedlifedata.com/resource/pubmed/chemical/Pertussis Toxin,
http://linkedlifedata.com/resource/pubmed/chemical/Phosphotransferases (Alcohol Group...,
http://linkedlifedata.com/resource/pubmed/chemical/Protein Precursors,
http://linkedlifedata.com/resource/pubmed/chemical/Psap protein, rat,
http://linkedlifedata.com/resource/pubmed/chemical/Saposins,
http://linkedlifedata.com/resource/pubmed/chemical/Sphingolipids,
http://linkedlifedata.com/resource/pubmed/chemical/Virulence Factors, Bordetella,
http://linkedlifedata.com/resource/pubmed/chemical/sphingosine kinase
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pubmed:status |
MEDLINE
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pubmed:month |
Feb
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pubmed:issn |
0892-6638
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
15
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
467-74
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pubmed:dateRevised |
2009-11-19
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pubmed:meshHeading |
pubmed-meshheading:11156962-Adrenal Gland Neoplasms,
pubmed-meshheading:11156962-Animals,
pubmed-meshheading:11156962-Apoptosis,
pubmed-meshheading:11156962-Cell Cycle,
pubmed-meshheading:11156962-DNA, Neoplasm,
pubmed-meshheading:11156962-Enzyme Activation,
pubmed-meshheading:11156962-Enzyme Inhibitors,
pubmed-meshheading:11156962-Flavonoids,
pubmed-meshheading:11156962-G0 Phase,
pubmed-meshheading:11156962-G1 Phase,
pubmed-meshheading:11156962-Glycoproteins,
pubmed-meshheading:11156962-Milk,
pubmed-meshheading:11156962-Mitogen-Activated Protein Kinases,
pubmed-meshheading:11156962-PC12 Cells,
pubmed-meshheading:11156962-Pertussis Toxin,
pubmed-meshheading:11156962-Pheochromocytoma,
pubmed-meshheading:11156962-Phosphotransferases (Alcohol Group Acceptor),
pubmed-meshheading:11156962-Protein Precursors,
pubmed-meshheading:11156962-Rats,
pubmed-meshheading:11156962-Saposins,
pubmed-meshheading:11156962-Sphingolipids,
pubmed-meshheading:11156962-Virulence Factors, Bordetella
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pubmed:year |
2001
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pubmed:articleTitle |
Prosaposin treatment induces PC12 entry in the S phase of the cell cycle and prevents apoptosis: activation of ERKs and sphingosine kinase.
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pubmed:affiliation |
Dipartimento di Medicina Sperimentale e Patologia, Università 'La Sapienza' Roma, Rome, Italy. roberta.misasi@uniroma1.it
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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