|
rdf:type |
|
|
lifeskim:mentions |
|
|
pubmed:issue |
3-4
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|
pubmed:dateCreated |
1977-1-25
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|
pubmed:abstractText |
From cells and culture media of embryonic rat fibroblasts (1st subculture) the acid mucopolysaccharides were isolated and fractionated. The per cent calculation of the 6 fractions was based on the content of glucuronic acid. The cultures were maintained as follows: 0.5 X 10(6) to 3 X 10(6) cells were examined in Demeter flasks at pH 7.4 or 6.6; lactate concentration was enhanced to 100 mg%. The amount of each fraction was correlated with the cell density (linear regression). The pH-value and lactate concentration in connection with cell density proved to be important factors in the modification of the MPS distribution pattern.
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pubmed:language |
eng
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|
pubmed:journal |
|
|
pubmed:citationSubset |
IM
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|
pubmed:chemical |
|
|
pubmed:status |
MEDLINE
|
|
pubmed:issn |
0014-4908
|
|
pubmed:author |
|
|
pubmed:issnType |
Print
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|
pubmed:volume |
12
|
|
pubmed:owner |
NLM
|
|
pubmed:authorsComplete |
Y
|
|
pubmed:pagination |
149-58
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|
pubmed:dateRevised |
2009-11-11
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|
pubmed:meshHeading |
pubmed-meshheading:11111-Animals,
pubmed-meshheading:11111-Cells, Cultured,
pubmed-meshheading:11111-Chondroitin Sulfates,
pubmed-meshheading:11111-Dermatan Sulfate,
pubmed-meshheading:11111-Embryo, Mammalian,
pubmed-meshheading:11111-Fibroblasts,
pubmed-meshheading:11111-Glycosaminoglycans,
pubmed-meshheading:11111-Heparitin Sulfate,
pubmed-meshheading:11111-Hyaluronic Acid,
pubmed-meshheading:11111-Hydrogen-Ion Concentration,
pubmed-meshheading:11111-Lactates,
pubmed-meshheading:11111-Rats
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|
pubmed:year |
1976
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|
pubmed:articleTitle |
Acid mucopolysaccharides in fibroblast cultures. 1. Influence of cell density, pH-value and lactate concentration on the MPS distribution pattern.
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pubmed:publicationType |
Journal Article
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