| pubmed-article:11041553 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:11041553 | lifeskim:mentions | umls-concept:C0086418 | lld:lifeskim |
| pubmed-article:11041553 | lifeskim:mentions | umls-concept:C0007634 | lld:lifeskim |
| pubmed-article:11041553 | lifeskim:mentions | umls-concept:C0010453 | lld:lifeskim |
| pubmed-article:11041553 | lifeskim:mentions | umls-concept:C0401925 | lld:lifeskim |
| pubmed-article:11041553 | lifeskim:mentions | umls-concept:C1704242 | lld:lifeskim |
| pubmed-article:11041553 | lifeskim:mentions | umls-concept:C1171362 | lld:lifeskim |
| pubmed-article:11041553 | lifeskim:mentions | umls-concept:C0596902 | lld:lifeskim |
| pubmed-article:11041553 | lifeskim:mentions | umls-concept:C0017262 | lld:lifeskim |
| pubmed-article:11041553 | lifeskim:mentions | umls-concept:C1515670 | lld:lifeskim |
| pubmed-article:11041553 | lifeskim:mentions | umls-concept:C1160577 | lld:lifeskim |
| pubmed-article:11041553 | lifeskim:mentions | umls-concept:C1314939 | lld:lifeskim |
| pubmed-article:11041553 | pubmed:issue | 6 | lld:pubmed |
| pubmed-article:11041553 | pubmed:dateCreated | 2001-1-17 | lld:pubmed |
| pubmed-article:11041553 | pubmed:abstractText | HGT-1 is a human cell line sharing a number of physiological features with gastric parietal cells. HGT-1 cell monolayers were able to secrete H+ when stimulated with histamine (calculated external pH variation, deltapH(e) 0.46+/-0.05) as assessed using the impermeant, pH-sensitive fluorescence dye 8-hydroxypyrene-1,3,6-trisulphonic acid, trisodium salt (HPTS). Treatment with 100 microM omeprazole inhibited the histamine-induced apical acidification by about 60%. Intracellular pH (pH(i)) measurements using the fluorescent pH-sensitive dye 2',7'-bis-carboxyethyl-5(6)-carboxyfluorescein (BCECF) demonstrated the expression of a functional, omeprazole-sensitive H+/K+-pump. A monoclonal antibody directed against the alpha subunit of the H+/K+-ATPase immunoprecipitated a 95-kDa protein from HGT-1 cells and human stomach which corresponds to the expected molecular size of the native protein. HGT-1 cells were also positive for the anion exchanger AE2 that is expressed in gastric parietal cells. In addition, we identified a histamine- and pH(i)-sensitive Na+/H+ exchanger in HGT-1 cells, which might correspond to the functional expression of the NHE4 isoform that has been detected in gastric epithelial cells as well as in primary cultured parietal cells. HGT-1 cells therefore display the principal features of parietal cells and might represent an interesting cell culture model for studying the regulatory mechanisms involved in acid secretion. | lld:pubmed |
| pubmed-article:11041553 | pubmed:language | eng | lld:pubmed |
| pubmed-article:11041553 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11041553 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:11041553 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11041553 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11041553 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11041553 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11041553 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11041553 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11041553 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11041553 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11041553 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11041553 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11041553 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11041553 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:11041553 | pubmed:month | Oct | lld:pubmed |
| pubmed-article:11041553 | pubmed:issn | 0031-6768 | lld:pubmed |
| pubmed-article:11041553 | pubmed:author | pubmed-author:SveltoMM | lld:pubmed |
| pubmed-article:11041553 | pubmed:author | pubmed-author:ValentiGG | lld:pubmed |
| pubmed-article:11041553 | pubmed:author | pubmed-author:CasavolaVV | lld:pubmed |
| pubmed-article:11041553 | pubmed:author | pubmed-author:ProcinoGG | lld:pubmed |
| pubmed-article:11041553 | pubmed:author | pubmed-author:CarmosinoMM | lld:pubmed |
| pubmed-article:11041553 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:11041553 | pubmed:volume | 440 | lld:pubmed |
| pubmed-article:11041553 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:11041553 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:11041553 | pubmed:pagination | 871-80 | lld:pubmed |
| pubmed-article:11041553 | pubmed:dateRevised | 2006-11-15 | lld:pubmed |
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| pubmed-article:11041553 | pubmed:year | 2000 | lld:pubmed |
| pubmed-article:11041553 | pubmed:articleTitle | The cultured human gastric cells HGT-1 express the principal transporters involved in acid secretion. | lld:pubmed |
| pubmed-article:11041553 | pubmed:affiliation | Dipartimento di Fisiologia Generale ed Ambientale, Bari, Italy. | lld:pubmed |
| pubmed-article:11041553 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:11041553 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
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| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:11041553 | lld:pubmed |
