Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
6
pubmed:dateCreated
2001-1-17
pubmed:abstractText
HGT-1 is a human cell line sharing a number of physiological features with gastric parietal cells. HGT-1 cell monolayers were able to secrete H+ when stimulated with histamine (calculated external pH variation, deltapH(e) 0.46+/-0.05) as assessed using the impermeant, pH-sensitive fluorescence dye 8-hydroxypyrene-1,3,6-trisulphonic acid, trisodium salt (HPTS). Treatment with 100 microM omeprazole inhibited the histamine-induced apical acidification by about 60%. Intracellular pH (pH(i)) measurements using the fluorescent pH-sensitive dye 2',7'-bis-carboxyethyl-5(6)-carboxyfluorescein (BCECF) demonstrated the expression of a functional, omeprazole-sensitive H+/K+-pump. A monoclonal antibody directed against the alpha subunit of the H+/K+-ATPase immunoprecipitated a 95-kDa protein from HGT-1 cells and human stomach which corresponds to the expected molecular size of the native protein. HGT-1 cells were also positive for the anion exchanger AE2 that is expressed in gastric parietal cells. In addition, we identified a histamine- and pH(i)-sensitive Na+/H+ exchanger in HGT-1 cells, which might correspond to the functional expression of the NHE4 isoform that has been detected in gastric epithelial cells as well as in primary cultured parietal cells. HGT-1 cells therefore display the principal features of parietal cells and might represent an interesting cell culture model for studying the regulatory mechanisms involved in acid secretion.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Oct
pubmed:issn
0031-6768
pubmed:author
pubmed:issnType
Print
pubmed:volume
440
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
871-80
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed-meshheading:11041553-1-Methyl-3-isobutylxanthine, pubmed-meshheading:11041553-Amiloride, pubmed-meshheading:11041553-Antibodies, Monoclonal, pubmed-meshheading:11041553-Cell Line, pubmed-meshheading:11041553-Coloring Agents, pubmed-meshheading:11041553-Enzyme Inhibitors, pubmed-meshheading:11041553-Fluoresceins, pubmed-meshheading:11041553-Fluorescent Antibody Technique, pubmed-meshheading:11041553-Gastric Acid, pubmed-meshheading:11041553-H(+)-K(+)-Exchanging ATPase, pubmed-meshheading:11041553-Histamine, pubmed-meshheading:11041553-Humans, pubmed-meshheading:11041553-Hydrogen-Ion Concentration, pubmed-meshheading:11041553-Immunosorbent Techniques, pubmed-meshheading:11041553-Kinetics, pubmed-meshheading:11041553-Models, Biological, pubmed-meshheading:11041553-Omeprazole, pubmed-meshheading:11041553-Parietal Cells, Gastric, pubmed-meshheading:11041553-Sodium, pubmed-meshheading:11041553-Tight Junctions
pubmed:year
2000
pubmed:articleTitle
The cultured human gastric cells HGT-1 express the principal transporters involved in acid secretion.
pubmed:affiliation
Dipartimento di Fisiologia Generale ed Ambientale, Bari, Italy.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't