Linked Life Data

10914546

Source:http://linkedlifedata.com/resource/pubmed/id/10914546

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
7
pubmed:dateCreated
2000-8-10
pubmed:abstractText
The t(12;21)(p13;q22) fusion gene is the most frequent genetic lesion described in precursor B cell acute lymphoblastic leukemia (ALL) of childhood occurring in a quarter of cases. This gene rearrangement is associated with a good outcome presenting a high response rate to chemotherapy. In spite of its potential clinical relevance, the t(12;21) translocation usually goes undetected with conventional cytogenetic procedures. In the present study we utilized an objective flow cytometric approach (multiparametric quantitative analysis) for the phenotypic characterization of this type of ALL. We studied a total of 74 precursor B-ALL children, including 21 t(12;21)+ and 53 t(12;21)- cases. Our results show that the t(12;21)(p13;q22)+ ALLs display a higher intensity of CD10 (P = 0.0016) and HLADR (P = 0.005) expression together with lower levels of the CD20 (P = 0.01), CD45 (P = 0.01), CD135 (P = 0.003) and CD34 (P = 0.03) antigens as compared to the t(12;21) cases. Moreover, as regards CD34 expression, we observed a more heterogeneous antigen expression within individual patients with higher coefficients of variation (median of 202 vs 88, P = 0.0001). A multi-variate analysis disclosed that with the immunophenotypic approach used identification of t(12;21)+ cases can be achieved with a sensitivity of 86% and a specificity of 100%. We conclude that childhood precursor B-ALL carrying the t(12;21) translocation display characteristic phenotypic features which could provide a rapid, simple, sensitive and specific screening method to select for those cases that should undergo confirmatory molecular analysis.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jul
pubmed:issn
0887-6924
pubmed:author
pubmed:issnType
Print
pubmed:volume
14
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1225-31
pubmed:dateRevised
2007-11-15
pubmed:meshHeading
pubmed-meshheading:10914546-Antigens, CD, pubmed-meshheading:10914546-Child, pubmed-meshheading:10914546-Chromosomes, Human, Pair 11, pubmed-meshheading:10914546-Chromosomes, Human, Pair 12, pubmed-meshheading:10914546-Chromosomes, Human, Pair 21, pubmed-meshheading:10914546-Chromosomes, Human, Pair 4, pubmed-meshheading:10914546-Core Binding Factor Alpha 2 Subunit, pubmed-meshheading:10914546-Flow Cytometry, pubmed-meshheading:10914546-Genotype, pubmed-meshheading:10914546-HLA-DR Antigens, pubmed-meshheading:10914546-Humans, pubmed-meshheading:10914546-Immunophenotyping, pubmed-meshheading:10914546-Multivariate Analysis, pubmed-meshheading:10914546-Neoplastic Stem Cells, pubmed-meshheading:10914546-Oncogene Proteins, Fusion, pubmed-meshheading:10914546-Ploidies, pubmed-meshheading:10914546-Precursor B-Cell Lymphoblastic Leukemia-Lymphoma, pubmed-meshheading:10914546-Precursor Cell Lymphoblastic Leukemia-Lymphoma, pubmed-meshheading:10914546-Reverse Transcriptase Polymerase Chain Reaction, pubmed-meshheading:10914546-Sensitivity and Specificity, pubmed-meshheading:10914546-Translocation, Genetic, pubmed-meshheading:10914546-Tumor Markers, Biological
pubmed:year
2000
pubmed:articleTitle
Quantitative multiparametric immunophenotyping in acute lymphoblastic leukemia: correlation with specific genotype. I. ETV6/AML1 ALLs identification.
pubmed:affiliation
Clinica Oncoemtologica Pediatrica, Dipartimento di Pediatra, Università di Padova, Italy.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't