|
rdf:type |
|
|
lifeskim:mentions |
umls-concept:C0013138,
umls-concept:C0017337,
umls-concept:C0205087,
umls-concept:C0243045,
umls-concept:C1514873,
umls-concept:C1527148,
umls-concept:C1533107,
umls-concept:C1546857,
umls-concept:C1556066,
umls-concept:C1619636,
umls-concept:C2700640
|
|
pubmed:issue |
1
|
|
pubmed:dateCreated |
2000-5-31
|
|
pubmed:abstractText |
The pulse of ecdysone that triggers Drosophila metamorphosis activates six early genes in a primary response made visible by polytene chromosome puffs. The secondary response is detected by the induction of over 100 late puffs, only a few of which have been subject to molecular genetic analysis. We present a molecular and mutational analysis of the L63 gene responsible for the late puff at 63E. This gene contains overlapping L63A, B, and C transcription units of which the A unit encodes two isoforms and the B unit three. The C unit, which exhibits little activity, encodes one of the B isoforms. Evidence that L63B, but not L63A, transcription is ecdysone responsive derives from their developmental transcription profiles and from P-element mutagenesis showing that ecdysone induction of the 63E puff requires sequences adjacent to the 5' end of L63B but not those adjacent to the 5' end of L63A. L63-specific lethal mutations showed that L63 is required not only for metamorphosis, but also maternally and for embryonic and larval development. The L63 proteins contain a common C-terminal 294-aa sequence that is 71% identical to the CDK sequence of the murine PFTAIRE protein. In vivo tests of L63 proteins altered by site-directed mutagenesis showed that they exhibit CDK functions. L63 proteins are widely distributed among late larval and prepupal tissues and are unlikely to be involved in cell cycle functions.
|
|
pubmed:language |
eng
|
|
pubmed:journal |
|
|
pubmed:citationSubset |
IM
|
|
pubmed:chemical |
|
|
pubmed:status |
MEDLINE
|
|
pubmed:month |
May
|
|
pubmed:issn |
0012-1606
|
|
pubmed:author |
|
|
pubmed:copyrightInfo |
Copyright 2000 Academic Press.
|
|
pubmed:issnType |
Print
|
|
pubmed:day |
1
|
|
pubmed:volume |
221
|
|
pubmed:owner |
NLM
|
|
pubmed:authorsComplete |
Y
|
|
pubmed:pagination |
23-40
|
|
pubmed:dateRevised |
2009-11-19
|
|
pubmed:meshHeading |
pubmed-meshheading:10772789-Amino Acid Sequence,
pubmed-meshheading:10772789-Animals,
pubmed-meshheading:10772789-Cell Cycle,
pubmed-meshheading:10772789-Chromosome Mapping,
pubmed-meshheading:10772789-Chromosomes, Artificial, Yeast,
pubmed-meshheading:10772789-Cloning, Molecular,
pubmed-meshheading:10772789-Cyclin-Dependent Kinases,
pubmed-meshheading:10772789-Drosophila,
pubmed-meshheading:10772789-Drosophila Proteins,
pubmed-meshheading:10772789-Ecdysone,
pubmed-meshheading:10772789-Gene Expression Regulation, Developmental,
pubmed-meshheading:10772789-Genes, Insect,
pubmed-meshheading:10772789-In Situ Hybridization,
pubmed-meshheading:10772789-Insect Proteins,
pubmed-meshheading:10772789-Metamorphosis, Biological,
pubmed-meshheading:10772789-Molecular Sequence Data,
pubmed-meshheading:10772789-Mutagenesis, Site-Directed,
pubmed-meshheading:10772789-Phenotype,
pubmed-meshheading:10772789-Protein Kinases,
pubmed-meshheading:10772789-RNA, Messenger,
pubmed-meshheading:10772789-Sequence Alignment,
pubmed-meshheading:10772789-Transcription Factors
|
|
pubmed:year |
2000
|
|
pubmed:articleTitle |
The L63 gene is necessary for the ecdysone-induced 63E late puff and encodes CDK proteins required for Drosophila development.
|
|
pubmed:affiliation |
Department of Biochemistry, Stanford University School of Medicine, Beckman Center, B300, Stanford, California 94305-5329, USA.
|
|
pubmed:publicationType |
Journal Article
|
