Linked Life Data

10690548

Source:http://linkedlifedata.com/resource/pubmed/id/10690548

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
2000-3-28
pubmed:abstractText
The expression of vascular endothelial growth factor (VEGF), a highly potent angiogenic molecule, is thought to be correlated with the development of colon cancer; however, direct evidence for a role of VEGF in metastasis is lacking. This study was designed to more directly establish the role of VEGF in the growth and metastasis of human colon cancer using a genetically engineered cancer cell line. A stable VEGF gene-transfected human colon cancer cell line, LoVo, was made by genetic manipulation using eukaryotic expression constructs designed to express the complete VEGF121 cDNA in the sense orientation. Transfected clones were screened for VEGF121 mRNA expression by Northern blot analysis and for VEGF121 protein expression by Western blot analysis. Consequently, we obtained S17 cells that expressed a high level of both VEGF mRNA and VEGF protein. A vector-transfected clone (V7 cell) was used as a control. The experiment with the dorsal air sac method revealed that S17 cells elicited a stronger directional out-growth of capillaries than V7 cells. S17 cells formed faster-growing tumors than did V7 cells when xenografted s.c. into nude mice, although there was no significant difference in their in vitro proliferation. Tumors derived from S17 cells had more vascularity, as assessed by counting capillary vessels after staining with factor VIII, than did tumors derived from V7 cells (P < 0.05). With regard to the metastatic potential, S17 cells exhibited a higher capacity for both hepatic metastasis after the splenic portal inoculation and peritoneal dissemination after i.p. injection than did V7 cells. However, S17 cells showed no apparent metastasis, despite their rapid growth after orthotopic implantation. In conclusion, the present study showed clearly that VEGF plays an important role in cancer growth due to stimulation of angiogenesis by accelerating cell growth after reaching the target organs.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Feb
pubmed:issn
1078-0432
pubmed:author
pubmed:issnType
Print
pubmed:volume
6
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
622-30
pubmed:dateRevised
2007-5-2
pubmed:meshHeading
pubmed-meshheading:10690548-Animals, pubmed-meshheading:10690548-Capillaries, pubmed-meshheading:10690548-Cell Division, pubmed-meshheading:10690548-Colonic Neoplasms, pubmed-meshheading:10690548-Endothelial Growth Factors, pubmed-meshheading:10690548-Humans, pubmed-meshheading:10690548-Liver Neoplasms, pubmed-meshheading:10690548-Lymphokines, pubmed-meshheading:10690548-Mice, pubmed-meshheading:10690548-Mice, Nude, pubmed-meshheading:10690548-Neoplasm Metastasis, pubmed-meshheading:10690548-Neovascularization, Pathologic, pubmed-meshheading:10690548-RNA, Messenger, pubmed-meshheading:10690548-Recombinant Proteins, pubmed-meshheading:10690548-Transfection, pubmed-meshheading:10690548-Transplantation, Heterologous, pubmed-meshheading:10690548-Tumor Cells, Cultured, pubmed-meshheading:10690548-Vascular Endothelial Growth Factor A, pubmed-meshheading:10690548-Vascular Endothelial Growth Factors
pubmed:year
2000
pubmed:articleTitle
Enhancement of angiogenesis, tumor growth, and metastasis by transfection of vascular endothelial growth factor into LoVo human colon cancer cell line.
pubmed:affiliation
Department of Internal Medicine, Graduate School of Medicine, Kyoto University, Japan. charly@kuhp.kyoto-u.ac.jp
pubmed:publicationType
Journal Article