Linked Life Data

10620131

Source:http://linkedlifedata.com/resource/pubmed/id/10620131

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
2000-3-9
pubmed:abstractText
Exposing human skin to ultraviolet radiation causes DNA damage, sunburn, immune alterations, and eventually, skin cancer. We wished to determine whether liposomes containing a DNA repair enzyme could prevent any of the acute effects of irradiation when applied after ultraviolet exposure. Fifteen human patients with a prior history of skin cancer were exposed to two minimal erythema doses of ultraviolet radiation on their buttock skin. Liposomes containing T4 endonuclease V or heat-inactivated enzyme were applied immediately and at 2, 4, and 5 h after ultraviolet irradiation. Transmission electron microscopy after anti-T4 endonuclease V-staining and immunogold labeling on biopsies taken at 6 h after ultraviolet exposure revealed that the enzyme was present within cells in the skin. Immunohistochemical DNA damage studies suggested a trend toward improved DNA repair at the active T4 endonuclease V liposome-treated test sites. Although the active T4 endonuclease V liposomes did not significantly affect the ultraviolet-induced erythema response and microscopic sunburn cell formation, they nearly completely prevented ultraviolet-induced upregulation of interleukin-10 and tumor necrosis factor-alpha RNA message and of interleukin-10 protein. These studies demonstrate that liposomes can be used for topical intracellular delivery of small proteins to human skin and suggest that liposomes containing DNA repair enzymes may provide a new avenue for photoprotection against some forms of ultraviolet-induced skin damage.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jan
pubmed:issn
0022-202X
pubmed:author
pubmed:issnType
Print
pubmed:volume
114
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
149-56
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed-meshheading:10620131-Administration, Topical, pubmed-meshheading:10620131-Adult, pubmed-meshheading:10620131-Aged, pubmed-meshheading:10620131-DNA Ligases, pubmed-meshheading:10620131-DNA Repair, pubmed-meshheading:10620131-Deoxyribonuclease (Pyrimidine Dimer), pubmed-meshheading:10620131-Drug Carriers, pubmed-meshheading:10620131-Endodeoxyribonucleases, pubmed-meshheading:10620131-Female, pubmed-meshheading:10620131-Humans, pubmed-meshheading:10620131-Interleukin-10, pubmed-meshheading:10620131-Keratinocytes, pubmed-meshheading:10620131-Langerhans Cells, pubmed-meshheading:10620131-Liposomes, pubmed-meshheading:10620131-Male, pubmed-meshheading:10620131-Microscopy, Electron, pubmed-meshheading:10620131-Middle Aged, pubmed-meshheading:10620131-Radiation-Protective Agents, pubmed-meshheading:10620131-Skin, pubmed-meshheading:10620131-Time Factors, pubmed-meshheading:10620131-Tumor Necrosis Factor-alpha, pubmed-meshheading:10620131-Ultraviolet Rays, pubmed-meshheading:10620131-Up-Regulation, pubmed-meshheading:10620131-Viral Proteins
pubmed:year
2000
pubmed:articleTitle
Topical treatment with liposomes containing T4 endonuclease V protects human skin in vivo from ultraviolet-induced upregulation of interleukin-10 and tumor necrosis factor-alpha.
pubmed:affiliation
Department of Dermatology, Karl Franzens University, Graz, Austria. peter.wolf@kfunigraz.ac.at
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, U.S. Gov't, Non-P.H.S., Research Support, Non-U.S. Gov't