Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
24
pubmed:dateCreated
2000-1-13
pubmed:databankReference
pubmed:abstractText
Two ATP-binding cassette (ABC) exporters are present in Pseudomonas fluorescens no. 33; one is the recently reported AprDEF system and the other is HasDEF, which exports a heme acquisition protein, HasA. The hasDEF genes were cloned by DNA hybridization with a DNA probe coding for the LipB protein, one of the components of the Serratia marcescens ABC exporter Lip system. P. fluorescens HasA showed sequence identity of 40 to 49% with HasA proteins from Pseudomonas aeruginosa and Serratia marcescens. The P. fluorescens Has exporter secreted HasA proteins from P. fluorescens and P. aeruginosa but not S. marcescens HasA in Escherichia coli, whereas the Has exporter from S. marcescens allowed secretion of all three HasA proteins. The P. fluorescens HasDEF system also promoted the secretion of the lipase and alkaline protease of P. fluorescens. Hybrid exporter analysis demonstrated that the HasD proteins, which are ABC proteins, are involved in the discrimination of export substrates. Chimeric HasA proteins containing both P. fluorescens and S. marcescens sequences were produced and tested for secretion through the Has exporters. The C-terminal region of HasA was shown to be involved in the secretion specificity of the P. fluorescens Has exporter.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/10601212-10215870, http://linkedlifedata.com/resource/pubmed/commentcorrection/10601212-10524213, http://linkedlifedata.com/resource/pubmed/commentcorrection/10601212-1282354, http://linkedlifedata.com/resource/pubmed/commentcorrection/10601212-1365398, http://linkedlifedata.com/resource/pubmed/commentcorrection/10601212-1904127, http://linkedlifedata.com/resource/pubmed/commentcorrection/10601212-1999392, http://linkedlifedata.com/resource/pubmed/commentcorrection/10601212-2112747, http://linkedlifedata.com/resource/pubmed/commentcorrection/10601212-2184029, http://linkedlifedata.com/resource/pubmed/commentcorrection/10601212-2995761, http://linkedlifedata.com/resource/pubmed/commentcorrection/10601212-3327753, http://linkedlifedata.com/resource/pubmed/commentcorrection/10601212-4598299, http://linkedlifedata.com/resource/pubmed/commentcorrection/10601212-6344016, http://linkedlifedata.com/resource/pubmed/commentcorrection/10601212-7592412, http://linkedlifedata.com/resource/pubmed/commentcorrection/10601212-7686882, http://linkedlifedata.com/resource/pubmed/commentcorrection/10601212-7774588, http://linkedlifedata.com/resource/pubmed/commentcorrection/10601212-7937909, http://linkedlifedata.com/resource/pubmed/commentcorrection/10601212-8021163, http://linkedlifedata.com/resource/pubmed/commentcorrection/10601212-8022281, http://linkedlifedata.com/resource/pubmed/commentcorrection/10601212-8071214, http://linkedlifedata.com/resource/pubmed/commentcorrection/10601212-8085787, http://linkedlifedata.com/resource/pubmed/commentcorrection/10601212-8132636, http://linkedlifedata.com/resource/pubmed/commentcorrection/10601212-8144462, http://linkedlifedata.com/resource/pubmed/commentcorrection/10601212-8226679, http://linkedlifedata.com/resource/pubmed/commentcorrection/10601212-8320370, http://linkedlifedata.com/resource/pubmed/commentcorrection/10601212-8866470, http://linkedlifedata.com/resource/pubmed/commentcorrection/10601212-8930911, http://linkedlifedata.com/resource/pubmed/commentcorrection/10601212-9171402, http://linkedlifedata.com/resource/pubmed/commentcorrection/10601212-9224868, http://linkedlifedata.com/resource/pubmed/commentcorrection/10601212-94251, http://linkedlifedata.com/resource/pubmed/commentcorrection/10601212-9535084, http://linkedlifedata.com/resource/pubmed/commentcorrection/10601212-9593292, http://linkedlifedata.com/resource/pubmed/commentcorrection/10601212-9680211
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
http://linkedlifedata.com/resource/pubmed/chemical/ATP-Binding Cassette Transporters, http://linkedlifedata.com/resource/pubmed/chemical/Adenosine Triphosphatases, http://linkedlifedata.com/resource/pubmed/chemical/Bacterial Outer Membrane Proteins, http://linkedlifedata.com/resource/pubmed/chemical/Bacterial Proteins, http://linkedlifedata.com/resource/pubmed/chemical/Carrier Proteins, http://linkedlifedata.com/resource/pubmed/chemical/HasA protein, Serratia marcescens, http://linkedlifedata.com/resource/pubmed/chemical/HasD protein, Serratia marcescens, http://linkedlifedata.com/resource/pubmed/chemical/HasE protein, Serratia marcescens, http://linkedlifedata.com/resource/pubmed/chemical/HasF protein, bacteria, http://linkedlifedata.com/resource/pubmed/chemical/Membrane Proteins, http://linkedlifedata.com/resource/pubmed/chemical/Recombinant Fusion Proteins
pubmed:status
MEDLINE
pubmed:month
Dec
pubmed:issn
0021-9193
pubmed:author
pubmed:issnType
Print
pubmed:volume
181
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
7545-51
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
1999
pubmed:articleTitle
Cloning and characterization of the Pseudomonas fluorescens ATP-binding cassette exporter, HasDEF, for the heme acquisition protein HasA.
pubmed:affiliation
Discovery Research Laboratory, Tanabe Seiyaku Co., Ltd., Yodogawa-ku, Osaka 532-8505, Japan.
pubmed:publicationType
Journal Article