Source:http://linkedlifedata.com/resource/pubmed/id/10517187
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
4
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| pubmed:dateCreated |
1999-11-3
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| pubmed:abstractText |
The hypothesis of the present work was that the pannus tissue overlying the articular hard tissues has an aggressive phenotype and contains the newly discovered collagenase-3 and its endogenous inducers and activators. We therefore analyzed the eventual presence of collagenase-3 and its regulation at the pannus-cartilage junction. Collagenase-3 mRNA (in situ hybridization) and enzyme protein (ABC and immunofluorescence staining) were found in the pannocytes in the pannus-hard tissue junction. Inflammatory round cells associated with the critical interface contained TNF-alpha and IL-1beta. These cytokines induced collagenase-3 secretion in cultured rheumatoid synovial fibroblasts. Procollagenase-3 activators, stromelysin-1, 72 kDa type IV collagenase/gelatinase and membrane-type 1-MMP, were also found in the pannus-hard tissue junction. Active collagenase-3 was inhibited with alendronate (IC50 = 500-750 microM). Collagenase-3, due to its substrate profile and local synthesis in a milieu favoring its activation, might play a major role in the degradation of cartilage type II and bone type I collagens. Alendronate, at concentrations attainable in vivo, is able to inhibit collagenase-3. This might offer an option to control collagenase-3-mediated tissue destruction in rheumatoid arthritis.
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| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Alendronate,
http://linkedlifedata.com/resource/pubmed/chemical/Collagenases,
http://linkedlifedata.com/resource/pubmed/chemical/Enzyme Inhibitors,
http://linkedlifedata.com/resource/pubmed/chemical/Interleukin-1,
http://linkedlifedata.com/resource/pubmed/chemical/MMP13 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Matrix Metalloproteinase 13,
http://linkedlifedata.com/resource/pubmed/chemical/Matrix Metalloproteinases,
http://linkedlifedata.com/resource/pubmed/chemical/Tumor Necrosis Factor-alpha
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| pubmed:status |
MEDLINE
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| pubmed:month |
Aug
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| pubmed:issn |
0945-053X
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| pubmed:author | |
| pubmed:issnType |
Print
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| pubmed:volume |
18
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| pubmed:owner |
NLM
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| pubmed:authorsComplete |
Y
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| pubmed:pagination |
401-12
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| pubmed:dateRevised |
2006-11-15
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| pubmed:meshHeading |
pubmed-meshheading:10517187-Adult,
pubmed-meshheading:10517187-Aged,
pubmed-meshheading:10517187-Alendronate,
pubmed-meshheading:10517187-Arthritis, Rheumatoid,
pubmed-meshheading:10517187-Blotting, Western,
pubmed-meshheading:10517187-Cartilage, Articular,
pubmed-meshheading:10517187-Collagenases,
pubmed-meshheading:10517187-Enzyme Inhibitors,
pubmed-meshheading:10517187-Exudates and Transudates,
pubmed-meshheading:10517187-Female,
pubmed-meshheading:10517187-Humans,
pubmed-meshheading:10517187-Immunohistochemistry,
pubmed-meshheading:10517187-Interleukin-1,
pubmed-meshheading:10517187-Male,
pubmed-meshheading:10517187-Matrix Metalloproteinase 13,
pubmed-meshheading:10517187-Matrix Metalloproteinases,
pubmed-meshheading:10517187-Middle Aged,
pubmed-meshheading:10517187-Synovial Membrane,
pubmed-meshheading:10517187-Tumor Necrosis Factor-alpha
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| pubmed:year |
1999
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| pubmed:articleTitle |
Collagenase-3 (MMP-13) and its activators in rheumatoid arthritis: localization in the pannus-hard tissue junction and inhibition by alendronate.
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| pubmed:affiliation |
Department of Medicine, Helsinki University Central Hospital, Finland. yrjo.konttinen@helsinki.fi
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| pubmed:publicationType |
Journal Article
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