Linked Life Data

10360962

Source:http://linkedlifedata.com/resource/pubmed/id/10360962

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
6
pubmed:dateCreated
1999-8-12
pubmed:abstractText
The human MHC class Ib gene HLA-G is transcribed and translated in different placental cell subpopulations during pregnancy. In addition to this restricted tissue distribution, HLA-G proteins were also recently detected in the thymus of HLA-G transgenic mice, as well as in some human thymic epithelial cells (TEC). There was a need to further define the phenotype of the HLA-G-expressing cells in the human thymus as well as the type of translated forms that they produce. Using several HLA-G-specific mAb and immunohistochemistry performed on cryosections of human thymi at different ages, we found that the HLA-G-expressing cells are present on medullary cells exhibiting the epithelial morphological type 6. Co-localization experiments performed by double or triple immunofluorescence staining demonstrate that these HLA-G-expressing cells express various cytokeratins, epithelial cell markers but not the CD83 dendritic cell marker. We further show by ELISA measurements that a subset of primary cultured human TEC also expresses soluble HLA-G. Therefore, HLA-G protein tissue distribution is not restricted solely to placental cells. A subpopulation of medullary TEC also expresses HLA-G both at their cell surface and in secreted form, raising the question of the functional significance of such MHC class Ib molecules. Whether thymic soluble and/or membrane-bound HLA-G contribute to inhibit NK cells or to a negative selection of autoreactive T cells which could be harmful in case of pregnancy and/or to a positive selection of viral peptides/HLA-G-restricted CD8(+) T cells remains to be demonstrated.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jun
pubmed:issn
0953-8178
pubmed:author
pubmed:issnType
Print
pubmed:volume
11
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
889-98
pubmed:dateRevised
2011-11-17
pubmed:meshHeading
pubmed-meshheading:10360962-Adolescent, pubmed-meshheading:10360962-Antigens, CD, pubmed-meshheading:10360962-Biological Markers, pubmed-meshheading:10360962-Child, pubmed-meshheading:10360962-Child, Preschool, pubmed-meshheading:10360962-Dendritic Cells, pubmed-meshheading:10360962-Epithelial Cells, pubmed-meshheading:10360962-HLA Antigens, pubmed-meshheading:10360962-HLA-G Antigens, pubmed-meshheading:10360962-Histocompatibility Antigens Class I, pubmed-meshheading:10360962-Humans, pubmed-meshheading:10360962-Immunoglobulins, pubmed-meshheading:10360962-Infant, Newborn, pubmed-meshheading:10360962-Membrane Glycoproteins, pubmed-meshheading:10360962-Membrane Proteins, pubmed-meshheading:10360962-Solubility, pubmed-meshheading:10360962-Thymus Gland, pubmed-meshheading:10360962-Transcription, Genetic
pubmed:year
1999
pubmed:articleTitle
HLA-G in the human thymus: a subpopulation of medullary epithelial but not CD83(+) dendritic cells expresses HLA-G as a membrane-bound and soluble protein.
pubmed:affiliation
INSERM U395, CHU Purpan, BP 3028, 31024 Toulouse Cedex 3, France.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't