Linked Life Data

10328683

Source:http://linkedlifedata.com/resource/pubmed/id/10328683

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:dateCreated
1999-5-13
pubmed:abstractText
In Bombyx mori, the female is the heterogametic sex and the sex determining system is referred to as ZZ/ZW. In a previous study, we found that this insect does not show dosage compensation at the transcriptional level. To confirm the validity of our conclusion, we investigated whether or not another sex-linked gene is dosage compensated. To identify new Z-linked genes, total RNA from reciprocal hybrid females between the silkworm strains p50 and C108 was compared using the differential display technique. Nine cDNA fragments corresponding to several differentially expressed mRNAs were cloned and sequenced. The analysis of nucleotide sequence polymorphisms confirmed that one of these cDNAs, ZDD4, originated from the Z chromosome. The amino acid sequence deduced from ZDD4 has homology with kettin, a modular protein in the Z-disc of Drosophila melanogaster muscles. On immunoblots of Bombyx larval muscle proteins a polypeptide of 380 kDa was labelled with antibody to the ZDD4 peptide. We considered that the gene corresponding to ZDD4 encodes a kettin homologue in the silkworm, and denote it as Bm kettin. By a three-point cross, Bm kettin was mapped at 40.0 CM on the Z chromosome. Southern blot analysis revealed that Bm kettin was present at one copy in the genome. Northern blot analysis showed that Bm Kettin mRNA was 9.1kb in length, and that the level of the mRNA in males was two times greater than that of females. Taken together with our previous observations, the present data suggest that lack of dosage compensation is a general rule in B. mori. Moreover, the twofold difference in Bm kettin expression between males and females may help explain the sexual difference in the wing flapping activity observed in some groups of Lepidoptera.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Feb
pubmed:issn
0018-067X
pubmed:author
pubmed:issnType
Print
pubmed:volume
82 ( Pt 2)
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
170-9
pubmed:dateRevised
2010-11-18
pubmed:meshHeading
pubmed:year
1999
pubmed:articleTitle
Bm kettin, homologue of the Drosophila kettin gene, is located on the Z chromosome in Bombyx mori and is not dosage compensated.
pubmed:affiliation
Graduate School of Agricultural and Life Sciences, University of Tokyo, Japan.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't