Source:http://linkedlifedata.com/resource/pubmed/id/10197586
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
7 Suppl
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| pubmed:dateCreated |
1999-4-22
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| pubmed:abstractText |
Pax-5 codes for the transcription factor BSAP, which plays an important role in midbrain patterning, B cell development, and lymphoma formation. Pax-5 is known to control gene expression by recognizing its target genes via the NH2-terminal paired domain and by regulating transcription through a COOH-terminal regulatory module consisting of activating and inhibitory sequences. The central region of Pax-5 contains a sequence with significant homology to the first alpha-helix of the paired-type homeodomain. This partial homeodomain has been highly conserved throughout vertebrate evolution because it is found not only in Pax-5 but also in the related Pax-2 and Pax-8 members of the same Pax subfamily. Here we report that the partial homeodomain binds the TATA-binding protein (TBP) and retinoblastoma (Rb) gene product. Both TBP and Rb were shown by coimmunoprecipitation experiments to directly associate with Pax-5 in vivo. The conserved core domain of TBP and the pocket region as well as COOH-terminal sequences of Rb are required for interaction with the partial homeodomain of Pax-5 in in vitro binding assays. Furthermore, Pax-5 was specifically bound only by the underphosphorylated form of Rb. These data indicate that Pax-5 is able to contact the basal transcription machinery through the TBP-containing initiation factor TFIID, and that its activity can be controlled by the cell cycle-regulated association with Rb.
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| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/B-Cell-Specific Activator Protein,
http://linkedlifedata.com/resource/pubmed/chemical/DNA-Binding Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Homeodomain Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Nuclear Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/PAX5 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Retinoblastoma Protein,
http://linkedlifedata.com/resource/pubmed/chemical/Transcription Factors
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| pubmed:status |
MEDLINE
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| pubmed:month |
Apr
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| pubmed:issn |
0008-5472
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| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:day |
1
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| pubmed:volume |
59
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| pubmed:owner |
NLM
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| pubmed:authorsComplete |
Y
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| pubmed:pagination |
1716s-1724s; discussion 1724s-1725s
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| pubmed:dateRevised |
2006-11-15
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| pubmed:meshHeading |
pubmed-meshheading:10197586-Amino Acid Sequence,
pubmed-meshheading:10197586-Animals,
pubmed-meshheading:10197586-B-Cell-Specific Activator Protein,
pubmed-meshheading:10197586-COS Cells,
pubmed-meshheading:10197586-Chromosome Deletion,
pubmed-meshheading:10197586-Conserved Sequence,
pubmed-meshheading:10197586-DNA-Binding Proteins,
pubmed-meshheading:10197586-Homeodomain Proteins,
pubmed-meshheading:10197586-Humans,
pubmed-meshheading:10197586-Molecular Sequence Data,
pubmed-meshheading:10197586-Nuclear Proteins,
pubmed-meshheading:10197586-Phosphorylation,
pubmed-meshheading:10197586-Protein Binding,
pubmed-meshheading:10197586-Retinoblastoma Protein,
pubmed-meshheading:10197586-Sequence Homology, Amino Acid,
pubmed-meshheading:10197586-TATA Box,
pubmed-meshheading:10197586-Transcription Factors,
pubmed-meshheading:10197586-Tumor Cells, Cultured
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| pubmed:year |
1999
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| pubmed:articleTitle |
The partial homeodomain of the transcription factor Pax-5 (BSAP) is an interaction motif for the retinoblastoma and TATA-binding proteins.
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| pubmed:affiliation |
Research Institute of Molecular Pathology, Vienna, Austria.
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| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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