| pubmed-article:10101232 | rdf:type | pubmed:Citation | lld:pubmed |
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| pubmed-article:10101232 | lifeskim:mentions | umls-concept:C0017337 | lld:lifeskim |
| pubmed-article:10101232 | lifeskim:mentions | umls-concept:C1514559 | lld:lifeskim |
| pubmed-article:10101232 | lifeskim:mentions | umls-concept:C0001497 | lld:lifeskim |
| pubmed-article:10101232 | lifeskim:mentions | umls-concept:C0022173 | lld:lifeskim |
| pubmed-article:10101232 | lifeskim:mentions | umls-concept:C0017262 | lld:lifeskim |
| pubmed-article:10101232 | lifeskim:mentions | umls-concept:C2349975 | lld:lifeskim |
| pubmed-article:10101232 | lifeskim:mentions | umls-concept:C2911684 | lld:lifeskim |
| pubmed-article:10101232 | lifeskim:mentions | umls-concept:C0185117 | lld:lifeskim |
| pubmed-article:10101232 | pubmed:issue | 1 | lld:pubmed |
| pubmed-article:10101232 | pubmed:dateCreated | 1999-5-21 | lld:pubmed |
| pubmed-article:10101232 | pubmed:databankReference | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10101232 | pubmed:databankReference | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10101232 | pubmed:abstractText | NeuroD, a basic helix-loop-helix transcription factor, plays an important role in neuronal differentiation. A rat NeuroD cDNA was obtained by the aid of reverse transcription-polymerase chain reaction (RT-PCR) and ligated to an expression vector having a CMV promoter. Transfection of the NeuroD-expression plasmid into PC12 cells, a rat pheochromocytoma cell line, induced morphological changes featured by neurite-like processes and synapse-like structures without a differentiation-inducing reagent such as NGF. In the transfected cells, the overproduced NeuroD was detected by Western blot analysis, and the expression of the gene encoding mid-sized neurofilaments, a neuron-specific marker, was demonstrated by RT-PCR. Adenylate kinase isozyme 1 (AK1) is an enzyme involved in the homeostasis of energy metabolism and appears specifically in neuronal cells during differentiation. The CAT reporter assay of the 5'-flanking region of the AK1 gene suggests that NeuroD activates the AK1 expression through E-boxes in the promoter region. RT-PCR analysis indicated the enhanced level of AK1 mRNA in NeuroD-producing PC12 cells. Electrophoretic mobility shift assays demonstrated that NeuroD was able to interact with a proximal E-box element of the AK1 promoter. The results indicated that NeuroD promoted the PC12 cells to differentiate into neuron-like cells with concomitant activation of the target genes including the AK1 and the neurofilament genes. | lld:pubmed |
| pubmed-article:10101232 | pubmed:language | eng | lld:pubmed |
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| pubmed-article:10101232 | pubmed:citationSubset | IM | lld:pubmed |
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| pubmed-article:10101232 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:10101232 | pubmed:month | Apr | lld:pubmed |
| pubmed-article:10101232 | pubmed:issn | 0169-328X | lld:pubmed |
| pubmed-article:10101232 | pubmed:author | pubmed-author:NakazawaAA | lld:pubmed |
| pubmed-article:10101232 | pubmed:author | pubmed-author:NomaTT | lld:pubmed |
| pubmed-article:10101232 | pubmed:author | pubmed-author:YoonY SYS | lld:pubmed |
| pubmed-article:10101232 | pubmed:copyrightInfo | Copyright 1999 Elsevier Science B.V. | lld:pubmed |
| pubmed-article:10101232 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:10101232 | pubmed:day | 6 | lld:pubmed |
| pubmed-article:10101232 | pubmed:volume | 67 | lld:pubmed |
| pubmed-article:10101232 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:10101232 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:10101232 | pubmed:pagination | 53-63 | lld:pubmed |
| pubmed-article:10101232 | pubmed:dateRevised | 2009-9-4 | lld:pubmed |
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| pubmed-article:10101232 | pubmed:meshHeading | pubmed-meshheading:10101232... | lld:pubmed |
| pubmed-article:10101232 | pubmed:year | 1999 | lld:pubmed |
| pubmed-article:10101232 | pubmed:articleTitle | Overexpression of NeuroD in PC12 cells alters morphology and enhances expression of the adenylate kinase isozyme 1 gene. | lld:pubmed |
| pubmed-article:10101232 | pubmed:affiliation | Department of Biochemistry, Yamaguchi University, School of Medicine, Ube, Yamaguchi 755-8505, Japan. tnoma@po.cc.yamagucyhi-u.ac.jp | lld:pubmed |
| pubmed-article:10101232 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:10101232 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
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