Linked Life Data

10101232

Source:http://linkedlifedata.com/resource/pubmed/id/10101232

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1999-5-21
pubmed:databankReference
pubmed:abstractText
NeuroD, a basic helix-loop-helix transcription factor, plays an important role in neuronal differentiation. A rat NeuroD cDNA was obtained by the aid of reverse transcription-polymerase chain reaction (RT-PCR) and ligated to an expression vector having a CMV promoter. Transfection of the NeuroD-expression plasmid into PC12 cells, a rat pheochromocytoma cell line, induced morphological changes featured by neurite-like processes and synapse-like structures without a differentiation-inducing reagent such as NGF. In the transfected cells, the overproduced NeuroD was detected by Western blot analysis, and the expression of the gene encoding mid-sized neurofilaments, a neuron-specific marker, was demonstrated by RT-PCR. Adenylate kinase isozyme 1 (AK1) is an enzyme involved in the homeostasis of energy metabolism and appears specifically in neuronal cells during differentiation. The CAT reporter assay of the 5'-flanking region of the AK1 gene suggests that NeuroD activates the AK1 expression through E-boxes in the promoter region. RT-PCR analysis indicated the enhanced level of AK1 mRNA in NeuroD-producing PC12 cells. Electrophoretic mobility shift assays demonstrated that NeuroD was able to interact with a proximal E-box element of the AK1 promoter. The results indicated that NeuroD promoted the PC12 cells to differentiate into neuron-like cells with concomitant activation of the target genes including the AK1 and the neurofilament genes.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Apr
pubmed:issn
0169-328X
pubmed:author
pubmed:copyrightInfo
Copyright 1999 Elsevier Science B.V.
pubmed:issnType
Print
pubmed:day
6
pubmed:volume
67
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
53-63
pubmed:dateRevised
2009-9-4
pubmed:meshHeading
pubmed-meshheading:10101232-Adenylate Kinase, pubmed-meshheading:10101232-Animals, pubmed-meshheading:10101232-Basic Helix-Loop-Helix Transcription Factors, pubmed-meshheading:10101232-Blotting, Northern, pubmed-meshheading:10101232-DNA, Complementary, pubmed-meshheading:10101232-DNA Primers, pubmed-meshheading:10101232-Gene Expression Regulation, Enzymologic, pubmed-meshheading:10101232-Helix-Loop-Helix Motifs, pubmed-meshheading:10101232-Isoenzymes, pubmed-meshheading:10101232-Nerve Tissue Proteins, pubmed-meshheading:10101232-Neurites, pubmed-meshheading:10101232-Neurons, pubmed-meshheading:10101232-PC12 Cells, pubmed-meshheading:10101232-Promoter Regions, Genetic, pubmed-meshheading:10101232-RNA, Messenger, pubmed-meshheading:10101232-Rats, pubmed-meshheading:10101232-Reverse Transcriptase Polymerase Chain Reaction, pubmed-meshheading:10101232-Transcription Factors
pubmed:year
1999
pubmed:articleTitle
Overexpression of NeuroD in PC12 cells alters morphology and enhances expression of the adenylate kinase isozyme 1 gene.
pubmed:affiliation
Department of Biochemistry, Yamaguchi University, School of Medicine, Ube, Yamaguchi 755-8505, Japan. tnoma@po.cc.yamagucyhi-u.ac.jp
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't