Source:http://linkedlifedata.com/resource/pubmed/id/10098632
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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
1
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pubmed:dateCreated |
1999-6-29
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pubmed:abstractText |
The effects of bombesin (BB) on mitogen activated protein (MAP) kinase were investigated using non-small cell lung cancer (NSCLC) cells. By Western blot, both 42 and 44 kDalton forms of MAP kinase were present in NCI-H1299 and NCI-H838 cells. Addition of BB to NCI-H1299 cells resulted in phosphorylation of the MAP kinase substrate myelin basic protein (MBP). Phosphorylation of MBP was maximal 6 min after the addition of 10 nM BB to NCI-H1299 cells. Addition of gastrin releasing peptide (GRP) or GRP14-27 but not GRP1-16 to NCI-H 1299 cells caused MBP phosphorylation. The effects of BB were inhibited by BW2258U89, a BB receptor antagonist, and PD98059, a MAP kinase kinase inhibitor. Also, PD98059 inhibited the clonal growth of NCI-H1299 cells. These data suggest that MAP kinase may be an important regulatory enzyme in NSCLC.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:issn |
0196-9781
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
20
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
121-6
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pubmed:dateRevised |
2007-11-15
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pubmed:meshHeading |
pubmed-meshheading:10098632-Amino Acid Sequence,
pubmed-meshheading:10098632-Bombesin,
pubmed-meshheading:10098632-Calcium-Calmodulin-Dependent Protein Kinases,
pubmed-meshheading:10098632-Carcinoma, Non-Small-Cell Lung,
pubmed-meshheading:10098632-Gastrin-Releasing Peptide,
pubmed-meshheading:10098632-Humans,
pubmed-meshheading:10098632-Lung Neoplasms,
pubmed-meshheading:10098632-Molecular Sequence Data,
pubmed-meshheading:10098632-Phosphorylation,
pubmed-meshheading:10098632-Tumor Cells, Cultured
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pubmed:year |
1999
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pubmed:articleTitle |
Bombesin activates MAP kinase in non-small cell lung cancer cells.
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pubmed:affiliation |
Department of Ophthalmology, University of Maryland School of Medicine, Baltimore 21201, USA.
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pubmed:publicationType |
Journal Article
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