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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
5
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pubmed:dateCreated |
1999-4-15
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pubmed:databankReference |
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pubmed:abstractText |
5-Lipoxygenase (5LO) plays a pivotal role in cellular leukotriene synthesis. To identify proteins interacting with human 5LO, we used a two-hybrid approach to screen a human lung cDNA library. From a total of 1.5 x 10(7) yeast transformants, nine independent clones representing three different proteins were isolated and found to specifically interact with 5LO. Four 1.7- to 1.8-kb clones represented a 16-kDa protein named coactosin-like protein for its significant homology with coactosin, a protein found to be associated with actin in Dictyostelium discoideum. Coactosin-like protein thus may provide a link between 5LO and the cytoskeleton. Two other yeast clones of 1.5 kb encoded transforming growth factor (TGF) type beta receptor-I-associated protein 1 partial cDNA. TGF type beta receptor-I-associated protein 1 recently has been reported to associate with the activated form of the TGF beta receptor I and may be involved in the TGF beta-induced up-regulation of 5LO expression and activity observed in HL-60 and Mono Mac 6 cells. Finally, three identical 2.1-kb clones contained the partial cDNA of a human protein with high homology to a hypothetical helicase K12H4. 8 from Caenorhabditis elegans and consequently was named DeltaK12H4. 8 homologue. Analysis of the predicted amino acid sequence revealed the presence of a RNase III motif and a double-stranded RNA binding domain, indicative of a protein of nuclear origin. The identification of these 5LO-interacting proteins provides additional approaches to studies of the cellular functions of 5LO.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/10051563-1731317,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10051563-2820055,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10051563-3895158,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10051563-3903434,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10051563-3943125,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10051563-6301011,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10051563-7665580,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10051563-7738170,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10051563-7816797,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10051563-7906398,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10051563-7929073,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10051563-8293475,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10051563-8327471,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10051563-8514741,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10051563-8620530,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10051563-8654983,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10051563-8665944,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10051563-8978031,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10051563-9241229,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10051563-9326934,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10051563-9545258,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10051563-9660180,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10051563-9684751,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10051563-9813031
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Activin Receptors, Type I,
http://linkedlifedata.com/resource/pubmed/chemical/Arachidonate 5-Lipoxygenase,
http://linkedlifedata.com/resource/pubmed/chemical/Caenorhabditis elegans Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Carrier Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Endoribonucleases,
http://linkedlifedata.com/resource/pubmed/chemical/Fungal Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Microfilament Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Protein-Serine-Threonine Kinases,
http://linkedlifedata.com/resource/pubmed/chemical/Protozoan Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Transforming Growth...,
http://linkedlifedata.com/resource/pubmed/chemical/Recombinant Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Ribonuclease III,
http://linkedlifedata.com/resource/pubmed/chemical/TGF-beta type I receptor,
http://linkedlifedata.com/resource/pubmed/chemical/beta-Galactosidase,
http://linkedlifedata.com/resource/pubmed/chemical/coactosin protein, Dictyostelium...
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pubmed:status |
MEDLINE
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pubmed:month |
Mar
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pubmed:issn |
0027-8424
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
2
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pubmed:volume |
96
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1881-5
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pubmed:dateRevised |
2010-11-18
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pubmed:meshHeading |
pubmed-meshheading:10051563-Activin Receptors, Type I,
pubmed-meshheading:10051563-Amino Acid Sequence,
pubmed-meshheading:10051563-Animals,
pubmed-meshheading:10051563-Arachidonate 5-Lipoxygenase,
pubmed-meshheading:10051563-Base Sequence,
pubmed-meshheading:10051563-Caenorhabditis elegans,
pubmed-meshheading:10051563-Caenorhabditis elegans Proteins,
pubmed-meshheading:10051563-Carrier Proteins,
pubmed-meshheading:10051563-Cell Line,
pubmed-meshheading:10051563-Cloning, Molecular,
pubmed-meshheading:10051563-Dictyostelium,
pubmed-meshheading:10051563-Endoribonucleases,
pubmed-meshheading:10051563-Fungal Proteins,
pubmed-meshheading:10051563-Gene Library,
pubmed-meshheading:10051563-Genes, Reporter,
pubmed-meshheading:10051563-HL-60 Cells,
pubmed-meshheading:10051563-Humans,
pubmed-meshheading:10051563-Lung,
pubmed-meshheading:10051563-Microfilament Proteins,
pubmed-meshheading:10051563-Molecular Sequence Data,
pubmed-meshheading:10051563-Protein-Serine-Threonine Kinases,
pubmed-meshheading:10051563-Protozoan Proteins,
pubmed-meshheading:10051563-Receptors, Transforming Growth Factor beta,
pubmed-meshheading:10051563-Recombinant Proteins,
pubmed-meshheading:10051563-Ribonuclease III,
pubmed-meshheading:10051563-Saccharomyces cerevisiae,
pubmed-meshheading:10051563-Sequence Alignment,
pubmed-meshheading:10051563-Sequence Homology, Amino Acid,
pubmed-meshheading:10051563-beta-Galactosidase
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pubmed:year |
1999
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pubmed:articleTitle |
Interaction of 5-lipoxygenase with cellular proteins.
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pubmed:affiliation |
Department of Medical Biochemistry and Biophysics, Division of Chemistry II, Karolinska Institute, S-171 77 Stockholm, Sweden.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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