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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
2
|
| pubmed:dateCreated |
1999-3-11
|
| pubmed:abstractText |
Matrix-assisted laser desorption/ionization mass spectrometry of DNA and proteins, directly deposited on the poly(tetrafluoroethylene) (Teflon) surface, is demonstrated. For DNA analysis, this technique apparently produces a more homogeneous coverage of the matrix/DNA over the sample surface. Moreover, it enhances the sensitivity and salt tolerance. As described here, this technique can also achieve an excellent mass resolution, similar to that observed using a metal probe for DNA up to 62mer. We also examined the use of Teflon as a sample support for protein analysis since Teflon has been used as a transfer membrane. Less than 25 fmol of myoglobin has been detected with this technique. In addition, effective MALDI-TOF analysis of salt-contaminated protein samples can also be accomplished by loading the protein sample onto Teflon, followed by steps of washing away salts, adding the matrix, and desorbing sample directly from Teflon.
|
| pubmed:grant | |
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Jan
|
| pubmed:issn |
0003-2700
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:day |
15
|
| pubmed:volume |
71
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
518-21
|
| pubmed:dateRevised |
2007-11-14
|
| pubmed:meshHeading | |
| pubmed:year |
1999
|
| pubmed:articleTitle |
Use of poly(tetrafluoroethylene)s as a sample support for the MALDI-TOF analysis of DNA and proteins.
|
| pubmed:affiliation |
Department of Chemistry, Cleveland State University, Ohio 44115, USA.
|
| pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
|