Switch to
Predicate | Object |
---|---|
rdf:type | |
lifeskim:mentions | |
pubmed:issue |
4
|
pubmed:dateCreated |
1999-4-13
|
pubmed:abstractText |
Affinity-purified antibodies to oligopeptides derived from two different regions of the carboxyterminus and cytoplasmic loop or to the last 103 C-terminal amino acids of mouse connexin37 (Cx37) were used to characterize expression of this gap junctional protein in endothelium of several murine tissues. Cx37 was expressed in endothelium of large blood vessels in brain, liver, kidney, spleen, heart, and lung, but not in capillaries. In addition, weak Cx37 immuno-signals were observed in lung respiratory epithelium of small bronchi and in alveolar epithelial cells of bronchioli. The ratios of Cx37 protein to Cx37 mRNA in adult and embryonic kidney as well as skin were 29-303-fold larger than in lung, suggesting that Cx37 mRNA was translated at different efficiencies in kidney and skin versus lung. Cx37 protein was more abundant in embryonic kidney and lung than in the corresponding adult tissues. After differential centrifugation of plasma membrane fractions in sucrose gradients, we found that Cx37-containing gap junctions in lung were much smaller than Cx32 and Cx26 aggregates from liver. HeLa cells were transfected with mouse Cx37 cDNA. In these cells, mouse Cx37 protein was phosphorylated mainly at serine, less at tyrosine, and very little at threonine residues. Three conductance states were resolved at 110, 240, and 315 pS.
|
pubmed:language |
eng
|
pubmed:journal | |
pubmed:citationSubset |
IM
|
pubmed:chemical | |
pubmed:status |
MEDLINE
|
pubmed:month |
Dec
|
pubmed:issn |
0171-9335
|
pubmed:author | |
pubmed:issnType |
Print
|
pubmed:volume |
77
|
pubmed:owner |
NLM
|
pubmed:authorsComplete |
Y
|
pubmed:pagination |
313-22
|
pubmed:dateRevised |
2006-11-15
|
pubmed:meshHeading |
pubmed-meshheading:9930656-Animals,
pubmed-meshheading:9930656-Connexins,
pubmed-meshheading:9930656-Endothelium, Vascular,
pubmed-meshheading:9930656-Fluorescent Antibody Technique, Indirect,
pubmed-meshheading:9930656-HeLa Cells,
pubmed-meshheading:9930656-Humans,
pubmed-meshheading:9930656-Liver,
pubmed-meshheading:9930656-Lung,
pubmed-meshheading:9930656-Mice,
pubmed-meshheading:9930656-Mice, Inbred BALB C,
pubmed-meshheading:9930656-Myocardium,
pubmed-meshheading:9930656-Patch-Clamp Techniques,
pubmed-meshheading:9930656-Phosphorylation,
pubmed-meshheading:9930656-Rabbits,
pubmed-meshheading:9930656-Rats,
pubmed-meshheading:9930656-Tissue Distribution,
pubmed-meshheading:9930656-Transfection
|
pubmed:year |
1998
|
pubmed:articleTitle |
Characterization of the gap junction protein connexin37 in murine endothelium, respiratory epithelium, and after transfection in human HeLa cells.
|
pubmed:affiliation |
Institut für Genetik, Universität Bonn, Germany. o.traub@uni-bonn.de
|
pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|