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rdf:type |
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lifeskim:mentions |
umls-concept:C0010834,
umls-concept:C0013139,
umls-concept:C0017262,
umls-concept:C0029045,
umls-concept:C0033684,
umls-concept:C0185117,
umls-concept:C0449774,
umls-concept:C1423524,
umls-concept:C1427622,
umls-concept:C1514925,
umls-concept:C1521761,
umls-concept:C1706089,
umls-concept:C2911684
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pubmed:issue |
2
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pubmed:dateCreated |
1999-3-9
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pubmed:abstractText |
To study the expression of the I factor, a non-long-terminal-repeat retrotransposon responsible for I-R hybrid dysgenesis in Drosophila melanogaster, we have tagged the ORF1 protein (ORF1p) by inserting the HA epitope in its N-terminal region. In transgenic flies, this modification is compatible with a high rate of autonomous transposition and allows direct estimation of the transposition frequency. I factor transposes in the germline of females (SF) that are daughters from crosses between I strain males (which contain active copies of the I factor) and R strain females (which do not). We analyzed the expression pattern of ORF1p by indirect immunofluorescence. Its expression correlates with retrotransposition. During oogenesis ORF1p appears unexpectedly as a cytoplasmic product, which accumulates with a specific pattern into the oocyte. A comparison of the expression patterns under conditions that modify the transposing activity of the element clarifies some aspects of I-factor functioning in the transposition process.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/9927467-106029,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9927467-109354,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9927467-1330483,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9927467-1647020,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9927467-1698618,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9927467-1710982,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9927467-1905801,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9927467-2158161,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9927467-2430722,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9927467-2449095,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9927467-2554335,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9927467-2850048,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9927467-2851098,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9927467-2901954,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9927467-5432063,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9927467-6088060,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9927467-6289436,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9927467-7565607,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9927467-7813907,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9927467-7937070,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9927467-7958404,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9927467-8001156,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9927467-8036166,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9927467-8074994,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9927467-817964,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9927467-819401,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9927467-8224830,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9927467-8365558,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9927467-8380889,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9927467-8395003,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9927467-8599946,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9927467-8670818,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9927467-8791535,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9927467-8945517,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9927467-8945518,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9927467-9250689,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9927467-9312060,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9927467-9475738
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Feb
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pubmed:issn |
0016-6731
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
151
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
761-71
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:9927467-Amino Acid Sequence,
pubmed-meshheading:9927467-Animals,
pubmed-meshheading:9927467-Animals, Genetically Modified,
pubmed-meshheading:9927467-Base Sequence,
pubmed-meshheading:9927467-Drosophila Proteins,
pubmed-meshheading:9927467-Drosophila melanogaster,
pubmed-meshheading:9927467-Female,
pubmed-meshheading:9927467-Gene Expression Regulation,
pubmed-meshheading:9927467-Genes, Insect,
pubmed-meshheading:9927467-Insect Proteins,
pubmed-meshheading:9927467-Molecular Sequence Data,
pubmed-meshheading:9927467-Oocytes,
pubmed-meshheading:9927467-Retroelements
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pubmed:year |
1999
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pubmed:articleTitle |
High-frequency retrotransposition of a marked I factor in Drosophila melanogaster correlates with a dynamic expression pattern of the ORF1 protein in the cytoplasm of oocytes.
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pubmed:affiliation |
Centre de Génétique Moléculaire, CNRS, 91198 Gif sur Yvette Cedex, France.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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