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rdf:type |
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lifeskim:mentions |
umls-concept:C0008356,
umls-concept:C0028429,
umls-concept:C0042210,
umls-concept:C0123759,
umls-concept:C0442027,
umls-concept:C0442118,
umls-concept:C0871261,
umls-concept:C1423842,
umls-concept:C1442162,
umls-concept:C1521840,
umls-concept:C1524063,
umls-concept:C1550718,
umls-concept:C1704632,
umls-concept:C1706817,
umls-concept:C2911692
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pubmed:issue |
1
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pubmed:dateCreated |
1999-1-21
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pubmed:abstractText |
We have investigated the effects of IL-12 and cholera toxin (CT) on the immune response to tetanus toxoid (TT) given by intranasal or oral routes. CT inhibited IL-12-induced IFN-gamma secretion both in vivo and in vitro. Intranasal administration of IL-12 to mice nasally immunized with the combined vaccine of TT and CT resulted in increased TT-specific IgG2a and IgG3 Abs, while IgG1 and IgE Ab responses were markedly reduced. This shift of the CT-induced immune response toward Th1 type was associated with TT-specific CD4+ T cells secreting IFN-gamma and reduced levels of Th2-type cytokines (i.e., IL-4, IL-5, IL-6, and IL-10). In contrast, intranasal IL-12 enhanced the CT-induced serum IgG1 and IgE Ab responses in mice given the combined vaccine orally. IFN-gamma secretion by TT-specific CD4+ T cells was also enhanced; however, Th2-type cytokine responses were predominant. Mucosal secretory IgA responses to oral or nasal vaccines were not affected by intranasal IL-12. Thus, intranasal IL-12 delivery influences Th cell subset development in mucosal inductive sites that are dependent on the route of vaccine delivery.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
AIM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antibodies, Bacterial,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD4,
http://linkedlifedata.com/resource/pubmed/chemical/Cholera Toxin,
http://linkedlifedata.com/resource/pubmed/chemical/Cytokines,
http://linkedlifedata.com/resource/pubmed/chemical/Epitopes, T-Lymphocyte,
http://linkedlifedata.com/resource/pubmed/chemical/Interferon-gamma,
http://linkedlifedata.com/resource/pubmed/chemical/Interleukin-12,
http://linkedlifedata.com/resource/pubmed/chemical/Liposomes,
http://linkedlifedata.com/resource/pubmed/chemical/Tetanus Toxoid,
http://linkedlifedata.com/resource/pubmed/chemical/Vaccines, Combined
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pubmed:status |
MEDLINE
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pubmed:month |
Jan
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pubmed:issn |
0022-1767
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
1
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pubmed:volume |
162
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
114-21
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pubmed:dateRevised |
2008-11-21
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pubmed:meshHeading |
pubmed-meshheading:9886376-Administration, Intranasal,
pubmed-meshheading:9886376-Administration, Oral,
pubmed-meshheading:9886376-Animals,
pubmed-meshheading:9886376-Antibodies, Bacterial,
pubmed-meshheading:9886376-Antigens, CD4,
pubmed-meshheading:9886376-Cells, Cultured,
pubmed-meshheading:9886376-Cholera Toxin,
pubmed-meshheading:9886376-Cytokines,
pubmed-meshheading:9886376-Epitopes, T-Lymphocyte,
pubmed-meshheading:9886376-Interferon-gamma,
pubmed-meshheading:9886376-Interleukin-12,
pubmed-meshheading:9886376-Liposomes,
pubmed-meshheading:9886376-Mice,
pubmed-meshheading:9886376-Mice, Inbred C57BL,
pubmed-meshheading:9886376-Nasal Mucosa,
pubmed-meshheading:9886376-Spleen,
pubmed-meshheading:9886376-Tetanus Toxoid,
pubmed-meshheading:9886376-Th1 Cells,
pubmed-meshheading:9886376-Th2 Cells,
pubmed-meshheading:9886376-Vaccines, Combined
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pubmed:year |
1999
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pubmed:articleTitle |
Use of intranasal IL-12 to target predominantly Th1 responses to nasal and Th2 responses to oral vaccines given with cholera toxin.
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pubmed:affiliation |
Department of Microbiology, Immunobiology Vaccine Center, University of Alabama Medical Center, Birmingham 35294-2170, USA.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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