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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
23
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pubmed:dateCreated |
1999-2-11
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pubmed:abstractText |
Peptides selected to bind to hepatitis B virus (HBV) core protein block interaction with the long viral surface antigen (L-HBsAg) in vitro. High resolution electron cryomicroscopy showed that one such peptide binds at the tips of the spikes of the core protein shell. The peptides contain two basic residues; changing either of two acidic residues at the spike tip to an alanine greatly reduced the binding affinity. Transfection of hepatoma cells with a replication-competent HBV plasmid gave significantly reduced production of virus in the presence of peptide, in a dose-dependent manner. These experiments show that the interaction of L-HBsAg with core particles is critical for HBV assembly, and give proof of principle for its disruption in vivo by small molecules.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/9843489-1713264,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9843489-1853575,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9843489-1992457,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9843489-2041079,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9843489-2463383,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9843489-2843594,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9843489-3015414,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9843489-3193246,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9843489-3740412,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9843489-3881765,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9843489-399329,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9843489-6606682,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9843489-7682645,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9843489-7784206,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9843489-7878002,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9843489-7892246,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9843489-8004680,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9843489-8194518,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9843489-8399211,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9843489-8740361,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9843489-9052786,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9843489-9052787,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9843489-9585237,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9843489-9642088
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Dec
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pubmed:issn |
0261-4189
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
1
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pubmed:volume |
17
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
6839-45
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:9843489-Antiviral Agents,
pubmed-meshheading:9843489-Binding Sites,
pubmed-meshheading:9843489-Cross-Linking Reagents,
pubmed-meshheading:9843489-Cryoelectron Microscopy,
pubmed-meshheading:9843489-Hepatitis B Core Antigens,
pubmed-meshheading:9843489-Hepatitis B Surface Antigens,
pubmed-meshheading:9843489-Hepatitis B virus,
pubmed-meshheading:9843489-Humans,
pubmed-meshheading:9843489-Mutagenesis,
pubmed-meshheading:9843489-Peptides,
pubmed-meshheading:9843489-Transfection,
pubmed-meshheading:9843489-Tumor Cells, Cultured,
pubmed-meshheading:9843489-Virus Assembly
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pubmed:year |
1998
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pubmed:articleTitle |
Peptides that block hepatitis B virus assembly: analysis by cryomicroscopy, mutagenesis and transfection.
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pubmed:affiliation |
Medical Research Council, Laboratory of Molecular Biology, Hills Road, Cambridge CB2 2QH, UK.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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