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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
1
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pubmed:dateCreated |
1998-12-10
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pubmed:abstractText |
Three amino-terminal-specific peptidic antibodies against cathepsin L were generated. These antibodies recognize in vitro processing products of procathepsin L in time-course-dependent fashion. Immunoblot analyses with these antibodies followed by immunoprecipitation with anti-cathepsin L antibody showed that the amino terminal amino acid sequences of intracellular cathepsin L are heterogeneous: the single chain form of cathepsin L starts with either EPLML, LKIPK or IPKSV, and the heavy chain of the two chain form with IPKSV. Percoll density gradient and fluorescence immunohistochemistry suggested that these three species of cathepsin L localize in the lysosomes where procathepsin L processing occurs.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Nov
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pubmed:issn |
0006-291X
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pubmed:author |
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pubmed:copyrightInfo |
Copyright 1998 Academic Press.
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pubmed:issnType |
Print
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pubmed:day |
9
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pubmed:volume |
252
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
202-7
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pubmed:dateRevised |
2009-11-19
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pubmed:meshHeading |
pubmed-meshheading:9813170-3T3 Cells,
pubmed-meshheading:9813170-Amino Acid Sequence,
pubmed-meshheading:9813170-Animals,
pubmed-meshheading:9813170-Cathepsin L,
pubmed-meshheading:9813170-Cathepsins,
pubmed-meshheading:9813170-Cell Transformation, Neoplastic,
pubmed-meshheading:9813170-Centrifugation, Density Gradient,
pubmed-meshheading:9813170-Cysteine Endopeptidases,
pubmed-meshheading:9813170-Endopeptidases,
pubmed-meshheading:9813170-Enzyme Precursors,
pubmed-meshheading:9813170-Genes, ras,
pubmed-meshheading:9813170-Kidney,
pubmed-meshheading:9813170-Kinetics,
pubmed-meshheading:9813170-Liver,
pubmed-meshheading:9813170-Mice,
pubmed-meshheading:9813170-Mice, Inbred C57BL,
pubmed-meshheading:9813170-Organ Specificity,
pubmed-meshheading:9813170-Protein Processing, Post-Translational,
pubmed-meshheading:9813170-Skin
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pubmed:year |
1998
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pubmed:articleTitle |
Multiple processing of procathepsin L to cathepsin L in vivo.
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pubmed:affiliation |
Department of Biochemistry, Juntendo University School of Medicine, 2-1-1 Hongo, Bunkyo-ku, Tokyo, 113, Japan. kishidoh@med.Juntendo.ac.jp
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pubmed:publicationType |
Journal Article
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