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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
11
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pubmed:dateCreated |
1998-12-11
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pubmed:abstractText |
A recent study showed that 43% of a population in the United Kingdom were seropositive for group C rotavirus. The higher than expected incidence may be due to limited diagnosis of acute human group C rotavirus infections because no routine test is available. Human group C rotavirus infections are routinely diagnosed by electron microscopy (EM) and a negative group A rotavirus enzyme-linked immunosorbent assay (ELISA) result. An antigen-detection ELISA was developed with hyperimmune antibodies raised to human group C rotavirus recombinant VP6 (Bristol strain) expressed in insect cells. The assay was used to screen fecal samples to determine the prevalence of group C rotavirus infection. Samples positive by ELISA were confirmed by EM, polyacrylamide gel electrophoresis of double-stranded RNA, or detection of the VP6 gene by reverse transcription-PCR. Retrospective analysis indicated a 1 to 2% detection rate of positivity among samples from patients with acute diarrhea.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/9774561-1316369,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9774561-1323577,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9774561-1326817,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9774561-1333486,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9774561-1651601,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9774561-3501633,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9774561-6169771,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9774561-7797945,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9774561-7852957,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9774561-8388891,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9774561-8634028,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9774561-8825710,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9774561-8940469,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9774561-9003623,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9774561-9131463,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9774561-9431910
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antibodies, Viral,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, Viral,
http://linkedlifedata.com/resource/pubmed/chemical/Capsid Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/DNA Primers,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Viral,
http://linkedlifedata.com/resource/pubmed/chemical/Recombinant Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/VP6 protein, Rotavirus
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pubmed:status |
MEDLINE
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pubmed:month |
Nov
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pubmed:issn |
0095-1137
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
36
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
3178-81
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:9774561-Animals,
pubmed-meshheading:9774561-Antibodies, Viral,
pubmed-meshheading:9774561-Antigens, Viral,
pubmed-meshheading:9774561-Base Sequence,
pubmed-meshheading:9774561-Capsid,
pubmed-meshheading:9774561-Capsid Proteins,
pubmed-meshheading:9774561-DNA Primers,
pubmed-meshheading:9774561-Diarrhea,
pubmed-meshheading:9774561-Electrophoresis, Polyacrylamide Gel,
pubmed-meshheading:9774561-Enzyme-Linked Immunosorbent Assay,
pubmed-meshheading:9774561-Feces,
pubmed-meshheading:9774561-Great Britain,
pubmed-meshheading:9774561-Humans,
pubmed-meshheading:9774561-Microscopy, Electron,
pubmed-meshheading:9774561-RNA, Viral,
pubmed-meshheading:9774561-Recombinant Proteins,
pubmed-meshheading:9774561-Reverse Transcriptase Polymerase Chain Reaction,
pubmed-meshheading:9774561-Rotavirus,
pubmed-meshheading:9774561-Rotavirus Infections,
pubmed-meshheading:9774561-Sensitivity and Specificity
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pubmed:year |
1998
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pubmed:articleTitle |
Enzyme-linked immunosorbent assay based on recombinant human group C rotavirus inner capsid protein (VP6) To detect human group C rotaviruses in fecal samples.
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pubmed:affiliation |
Public Health Laboratory, Southampton General Hospital, Southampton SO16 6YD, United Kingdom. vlaj@soton.ac.uk
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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