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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
4 Pt 2
|
| pubmed:dateCreated |
1998-11-19
|
| pubmed:abstractText |
Laminin is a major component of the extracellular matrix whose expression is regulated by growth factors. The laminin gamma1-chain promoter contains a newly identified transcriptional element denoted bcn-1 that is both active and inducible in mesangial cells. In this study, we explored activation of the bcn-1 element in other renal and nonrenal cells. Treatment of rat glomerular epithelial cells (GEC) with phorbol 12-myristate 13-acetate (PMA) increased activity of the bcn-1 transcriptional element, within the context of the native laminin gamma1-chain promoter or when cloned upstream of a heterologous promoter. Treatment of GEC with PMA induced nuclear DNA-binding activity, BCN-1, which was recognized by the bcn-1 motif in a gel shift assay. These results provide evidence that the bcn-1 motif and its cognate BCN-1 factor(s) may regulate transcription of the laminin gamma1-chain in GEC. The bcn-1 element and its cognate BCN-1 DNA-binding activity were also inducible in monkey kidney COS-7 and in human T cell Jurkat lines. SDS-PAGE of in situ ultraviolet cross-linked nucleoproteins from GEC, COS, and Jurkat cells revealed one major 110-115 kDa adduct in all three cell lines. These results demonstrate that the bcn-1 element is active in renal and nonrenal cells from different mammalian species where the same protein contributes to the inducible BCN-1 DNA-binding activity.
|
| pubmed:grant | |
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/DNA-Binding Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Laminin,
http://linkedlifedata.com/resource/pubmed/chemical/Nuclear Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger,
http://linkedlifedata.com/resource/pubmed/chemical/Tetradecanoylphorbol Acetate,
http://linkedlifedata.com/resource/pubmed/chemical/laminin gamma 1
|
| pubmed:status |
MEDLINE
|
| pubmed:month |
Oct
|
| pubmed:issn |
0002-9513
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
275
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
F518-26
|
| pubmed:dateRevised |
2008-11-21
|
| pubmed:meshHeading |
pubmed-meshheading:9755123-Animals,
pubmed-meshheading:9755123-Base Sequence,
pubmed-meshheading:9755123-Binding Sites,
pubmed-meshheading:9755123-COS Cells,
pubmed-meshheading:9755123-Cell Nucleus,
pubmed-meshheading:9755123-Cloning, Molecular,
pubmed-meshheading:9755123-Conserved Sequence,
pubmed-meshheading:9755123-DNA-Binding Proteins,
pubmed-meshheading:9755123-Epithelial Cells,
pubmed-meshheading:9755123-Humans,
pubmed-meshheading:9755123-Jurkat Cells,
pubmed-meshheading:9755123-Kidney Glomerulus,
pubmed-meshheading:9755123-Laminin,
pubmed-meshheading:9755123-Nuclear Proteins,
pubmed-meshheading:9755123-Promoter Regions, Genetic,
pubmed-meshheading:9755123-RNA, Messenger,
pubmed-meshheading:9755123-Rats,
pubmed-meshheading:9755123-Tetradecanoylphorbol Acetate,
pubmed-meshheading:9755123-Transcription, Genetic,
pubmed-meshheading:9755123-Transfection
|
| pubmed:year |
1998
|
| pubmed:articleTitle |
Inducible transcriptional activity of bcn-1 element from laminin gamma1-chain gene promoter in renal and nonrenal cells.
|
| pubmed:affiliation |
Department of Medicine, University of Washington, Seattle, Washington 98195, USA.
|
| pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
|