|
rdf:type |
|
|
lifeskim:mentions |
umls-concept:C0003250,
umls-concept:C0024518,
umls-concept:C0063356,
umls-concept:C0104998,
umls-concept:C0111208,
umls-concept:C0699040,
umls-concept:C1332709,
umls-concept:C1366561,
umls-concept:C1413237,
umls-concept:C1423536,
umls-concept:C1522634,
umls-concept:C1705431
|
|
pubmed:issue |
6
|
|
pubmed:dateCreated |
1998-10-6
|
|
pubmed:abstractText |
The identification of all CD28/CTLA-4 counterreceptors is critical to our understanding of this pivotal pathway of T cell activation. Clouding our understanding has been the reported discrepancies in expression and function of the B7-1 (CD80) molecule based upon the use of the BB1 vs other anti-B7-1 mAbs. To resolve this issue, we have cloned a BB1-binding molecule from the BB1+B7-1(-) NALM-6 pre-B cell line. Here, we demonstrate that this BB1-binding molecule is identical to the cell surface form of CD74 (MHC class II-associated invariant chain). CD74-transfected cells bound the BB1 mAb but not other anti-CD80 mAbs, CD28-Ig, or CTLA4Ig. Absorption and blocking experiments confirmed the reactivity of BB1 mAb with CD74. A region of weak homology was identified between CD74 and the region of B7-1 encoding the BB1 epitope. Therefore, the BB1 mAb binds to a protein distinct from B7-1, and this epitope is also present on the B7-1 protein. Many of the puzzling observations in the literature concerning the expression of human B7-1 are resolved by an understanding that BB1 staining is the summation of CD74 plus B7-1 expression. This observation requires the field to reconsider studies using BB1 mAb in the analysis of CD80 expression and function.
|
|
pubmed:grant |
|
|
pubmed:language |
eng
|
|
pubmed:journal |
|
|
pubmed:citationSubset |
AIM
|
|
pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antibodies, Blocking,
http://linkedlifedata.com/resource/pubmed/chemical/Antibodies, Monoclonal,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD28,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD80,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, Differentiation,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, Differentiation...,
http://linkedlifedata.com/resource/pubmed/chemical/CTLA-4 Antigen,
http://linkedlifedata.com/resource/pubmed/chemical/CTLA4 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Ctla4 protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/Histocompatibility Antigens Class II,
http://linkedlifedata.com/resource/pubmed/chemical/Immunoconjugates,
http://linkedlifedata.com/resource/pubmed/chemical/Immunoglobulin Fc Fragments,
http://linkedlifedata.com/resource/pubmed/chemical/Interferon-gamma,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger,
http://linkedlifedata.com/resource/pubmed/chemical/Recombinant Fusion Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/abatacept,
http://linkedlifedata.com/resource/pubmed/chemical/invariant chain
|
|
pubmed:status |
MEDLINE
|
|
pubmed:month |
Sep
|
|
pubmed:issn |
0022-1767
|
|
pubmed:author |
|
|
pubmed:issnType |
Print
|
|
pubmed:day |
15
|
|
pubmed:volume |
161
|
|
pubmed:owner |
NLM
|
|
pubmed:authorsComplete |
Y
|
|
pubmed:pagination |
2708-15
|
|
pubmed:dateRevised |
2011-11-17
|
|
pubmed:meshHeading |
pubmed-meshheading:9743327-3T3 Cells,
pubmed-meshheading:9743327-Amino Acid Sequence,
pubmed-meshheading:9743327-Animals,
pubmed-meshheading:9743327-Antibodies, Blocking,
pubmed-meshheading:9743327-Antibodies, Monoclonal,
pubmed-meshheading:9743327-Antibody Specificity,
pubmed-meshheading:9743327-Antigens, CD,
pubmed-meshheading:9743327-Antigens, CD28,
pubmed-meshheading:9743327-Antigens, CD80,
pubmed-meshheading:9743327-Antigens, Differentiation,
pubmed-meshheading:9743327-Antigens, Differentiation, B-Lymphocyte,
pubmed-meshheading:9743327-Binding Sites, Antibody,
pubmed-meshheading:9743327-CHO Cells,
pubmed-meshheading:9743327-COS Cells,
pubmed-meshheading:9743327-CTLA-4 Antigen,
pubmed-meshheading:9743327-Cloning, Molecular,
pubmed-meshheading:9743327-Cricetinae,
pubmed-meshheading:9743327-Histocompatibility Antigens Class II,
pubmed-meshheading:9743327-Humans,
pubmed-meshheading:9743327-Immunoconjugates,
pubmed-meshheading:9743327-Immunoglobulin Fc Fragments,
pubmed-meshheading:9743327-Interferon-gamma,
pubmed-meshheading:9743327-Keratinocytes,
pubmed-meshheading:9743327-Mice,
pubmed-meshheading:9743327-Molecular Sequence Data,
pubmed-meshheading:9743327-Phenotype,
pubmed-meshheading:9743327-Precursor B-Cell Lymphoblastic Leukemia-Lymphoma,
pubmed-meshheading:9743327-RNA, Messenger,
pubmed-meshheading:9743327-Recombinant Fusion Proteins,
pubmed-meshheading:9743327-Tumor Cells, Cultured
|
|
pubmed:year |
1998
|
|
pubmed:articleTitle |
The BB1 monoclonal antibody recognizes both cell surface CD74 (MHC class II-associated invariant chain) as well as B7-1 (CD80), resolving the question regarding a third CD28/CTLA-4 counterreceptor.
|
|
pubmed:affiliation |
Department of Adult Oncology, Dana-Farber Cancer Institute, Brigham and Women's Hospital, Boston, MA 02115, USA. gordon freeman@macmailgw.dfci.harvard.edu
|
|
pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
|
