Linked Life Data

9665798

Source:http://linkedlifedata.com/resource/pubmed/id/9665798

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1998-8-10
pubmed:abstractText
Trophoblasts cells which are derived from the outer layer of the blastocyst have developed mechanisms by which they can invade the uterus and tap into the maternal circulation. In contrast to tumor cell invasion trophoblast invasion is precisely regulated, being confined spatially to the uterus and temporally to early pregnancy. The invasive properties manifested by trophoblasts are made possible by the secretion of proteolytic enzymes which can degrade components of the extracellular matrix (ECM). A number of investigators have shown that the matrix metalloproteinases (MMPs) are important mediators of trophoblast invasion. The two type IV collagenases, MMP-2 and MMP-9, which specifically degrade type IV collagen and gelatins have been of particular interest in this respect. In this paper we examine the expression and regulation of MMPs and their inhibitors in a series of trophoblast continuous cell lines. These cell lines, ED27, ED31, ED77, and a choriocarcinoma cell line, BeWo, were initially characterized with respect to various properties, including cytokeratin, hCG, and hPL expression. We have looked at the expression of MMPs and their inhibitors in these cell lines and their in vitro invasive behavior. Using zymography and RT-PCR we show that the trophoblast cell lines produce both MMP-2 and MMP-9, while the BeWo produce only MMP-2. Using an in vitro invasion assay the trophoblast cell lines were shown to be capable of invading while the BeWo were unable to invade. These results suggest that expression of MMP-9 in these cells is crucial for invasion. We have also examined the regulation of MMP expression by cytokines and found that MMP-9 expression could be modulated by IL-1 beta in these cell lines. The data presented in this paper suggest that these trophoblast cell lines present an ideal model system to investigate the regulation of metalloproteinases in trophoblast invasion.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jul
pubmed:issn
0014-4827
pubmed:author
pubmed:issnType
Print
pubmed:day
10
pubmed:volume
242
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
18-26
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed-meshheading:9665798-Cell Line, pubmed-meshheading:9665798-Cell Movement, pubmed-meshheading:9665798-Chorionic Gonadotropin, pubmed-meshheading:9665798-Collagen, pubmed-meshheading:9665798-Cytokines, pubmed-meshheading:9665798-Drug Combinations, pubmed-meshheading:9665798-Extracellular Matrix, pubmed-meshheading:9665798-Female, pubmed-meshheading:9665798-Gene Expression Regulation, Enzymologic, pubmed-meshheading:9665798-Humans, pubmed-meshheading:9665798-Keratins, pubmed-meshheading:9665798-Laminin, pubmed-meshheading:9665798-Metalloendopeptidases, pubmed-meshheading:9665798-Placental Lactogen, pubmed-meshheading:9665798-Pregnancy, pubmed-meshheading:9665798-Protease Inhibitors, pubmed-meshheading:9665798-Proteoglycans, pubmed-meshheading:9665798-RNA, Messenger, pubmed-meshheading:9665798-Trophoblasts
pubmed:year
1998
pubmed:articleTitle
Expression of metalloproteinases and their inhibitors in human trophoblast continuous cell lines.
pubmed:affiliation
School of Biological Sciences, Dublin City University, Ireland. 94971153@tolka.dcu.ie
pubmed:publicationType
Journal Article