Switch to
| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
6
|
| pubmed:dateCreated |
1998-6-19
|
| pubmed:abstractText |
Anesthetics and sedatives contribute to postoperative immunosuppression. Interleukin-8 (IL-8) is a chemotactic and activating factor that mediates neutrophil adhesion and margination and is essential for host defense. We investigated the effect of anesthetics on isolated human polymorphonuclear leukocyte production of IL-8. Healthy human polymorphonuclear leukocytes were isolated using a single-step density gradient and stimulated with lipopolysaccharide in the presence of varying concentrations of propofol or midazolam for up to 20 h. IL-8 was measured in both culture supernatants and cell lysates using enzyme immunoassay, and IL-8 mRNA in cells was measured using Northern blotting and phosphorimaging. Data were analyzed using Kruskal-Wallis analysis of variance or the Mann-Whitney U-test as appropriate. Lipopolysaccharide increased extracellular accumulation of interleukin-8, which was suppressed by both propofol (P = 0.025) and midazolam (P = 0.028). However, intracellular IL-8 increased with exposure to lipopolysaccharide (P = 0.028) and remained increased with both anesthetics. Northern blot analysis also revealed increased IL-8 mRNA levels in the presence of both midazolam and propofol, which was confirmed by molecular imaging. These data strongly suggest that the anesthetics modulate transport or secretion of IL-8 protein from the cell. Suppression of IL-8 by anesthetics and sedatives may predispose postoperative and intensive care patients to infection. Implications: Anesthesia causes immune suppression and alters neutrophil function. We investigated the effect of propofol and midazolam on interleukin-8, a neutrophil chemotactic agent in human neutrophils. Both anesthetics decreased extracellular interleukin-8 accumulation, but intracellular levels and mRNA remained high. This suggests that propofol and midazolam alter interleukin-8 secretion from cells.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
AIM
|
| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Anesthetics, Intravenous,
http://linkedlifedata.com/resource/pubmed/chemical/Chemotactic Factors,
http://linkedlifedata.com/resource/pubmed/chemical/Hypnotics and Sedatives,
http://linkedlifedata.com/resource/pubmed/chemical/Interleukin-8,
http://linkedlifedata.com/resource/pubmed/chemical/Lipopolysaccharides,
http://linkedlifedata.com/resource/pubmed/chemical/Midazolam,
http://linkedlifedata.com/resource/pubmed/chemical/Propofol,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger
|
| pubmed:status |
MEDLINE
|
| pubmed:month |
Jun
|
| pubmed:issn |
0003-2999
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
86
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
1289-93
|
| pubmed:dateRevised |
2004-11-17
|
| pubmed:meshHeading |
pubmed-meshheading:9620522-Analysis of Variance,
pubmed-meshheading:9620522-Anesthetics, Intravenous,
pubmed-meshheading:9620522-Biological Transport,
pubmed-meshheading:9620522-Blotting, Northern,
pubmed-meshheading:9620522-Cell Adhesion,
pubmed-meshheading:9620522-Cell Survival,
pubmed-meshheading:9620522-Chemotactic Factors,
pubmed-meshheading:9620522-Chemotaxis, Leukocyte,
pubmed-meshheading:9620522-Humans,
pubmed-meshheading:9620522-Hypnotics and Sedatives,
pubmed-meshheading:9620522-Immune Tolerance,
pubmed-meshheading:9620522-Intensive Care,
pubmed-meshheading:9620522-Interleukin-8,
pubmed-meshheading:9620522-Lipopolysaccharides,
pubmed-meshheading:9620522-Midazolam,
pubmed-meshheading:9620522-Neutrophil Activation,
pubmed-meshheading:9620522-Neutrophils,
pubmed-meshheading:9620522-Opportunistic Infections,
pubmed-meshheading:9620522-Postoperative Complications,
pubmed-meshheading:9620522-Propofol,
pubmed-meshheading:9620522-RNA, Messenger,
pubmed-meshheading:9620522-Risk Factors
|
| pubmed:year |
1998
|
| pubmed:articleTitle |
The effect of midazolam and propofol on interleukin-8 from human polymorphonuclear leukocytes.
|
| pubmed:affiliation |
Academic Unit of Anaesthesia and Intensive Care, University of Aberdeen, Scotland. h.f.galley@abdn.ac.uk
|
| pubmed:publicationType |
Journal Article
|