Switch to
| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
5
|
| pubmed:dateCreated |
1998-7-31
|
| pubmed:abstractText |
The aim of this study was to evaluate the effect of dextran (Dx) 1 versus Dx 60 (molecular weights 1,000 and 60,000) on microvascular disturbances and tissue injury in striated muscle after ischemia/reperfusion (I/R). Experiments were performed using a 4 h pressure-induced ischemia model in the hamster dorsal skinfold chamber. Three groups (n=6) of animals received a continuous infusion (45 min, 3 microL/min) of either Dx 1 or Dx 60 (total dose 5 mg/kg) or saline solution beginning 15 min before reperfusion. Intravital fluorescence microscopy allowed for quantification of functional capillary density, leukocyte adherence, extravasation of fluorescein isothiocyanate-Dx, and nonviable (propidium-positive) cell count before ischemia and .5, 2, and 24 h after reperfusion. Experiments were terminated with tissue preservation for electron microscopy. Postischemic functional capillary density was significantly improved by Dx 60 (at 24 h, 88% vs. 51% in controls). In animals receiving postischemic Dx 1 or Dx 60, leukocyte adherence was significantly reduced (at .5 h, 44% and 58%, respectively) as compared with controls, whereas macromolecular extravasation was unchanged. Nonviable cell count was significantly decreased by both Dx fractions (at 24 h, Dx 1, 75%; Dx 60, 87%), indicating a reduction of tissue injury, which was also confirmed by electron microscopy. These results provide evidence that Dx 60 at 5 mg/kg attenuates I/R injury more effectively than Dx 1. Leukocytes play a major role in the development of I/R injury, but macromolecular extravasation does not always correlate with the leukocyte-endothelium interaction and the manifestation of I/R injury.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
May
|
| pubmed:issn |
1073-2322
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
9
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
345-51
|
| pubmed:dateRevised |
2006-11-15
|
| pubmed:meshHeading |
pubmed-meshheading:9617884-Animals,
pubmed-meshheading:9617884-Capillaries,
pubmed-meshheading:9617884-Cell Survival,
pubmed-meshheading:9617884-Cricetinae,
pubmed-meshheading:9617884-Dextrans,
pubmed-meshheading:9617884-Endothelium, Vascular,
pubmed-meshheading:9617884-Ischemia,
pubmed-meshheading:9617884-Leukocytes,
pubmed-meshheading:9617884-Mesocricetus,
pubmed-meshheading:9617884-Microcirculation,
pubmed-meshheading:9617884-Microscopy,
pubmed-meshheading:9617884-Microscopy, Electron,
pubmed-meshheading:9617884-Muscle, Skeletal,
pubmed-meshheading:9617884-Pressure,
pubmed-meshheading:9617884-Reperfusion,
pubmed-meshheading:9617884-Skin,
pubmed-meshheading:9617884-Time Factors,
pubmed-meshheading:9617884-Venules
|
| pubmed:year |
1998
|
| pubmed:articleTitle |
Impact of dextran on microvascular disturbances and tissue injury following ischemia/reperfusion in striated muscle.
|
| pubmed:affiliation |
Institute for Surgical Research, University of Munich, Germany.
|
| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|